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The effect of photoperiod on the haemolymph protein profile in the silkworm, Bombyx mori L.

Published online by Cambridge University Press:  19 September 2011

S. Janarthanan ,
K. M. Subbu Rathinam  and
M. Krishnan
S. Janarthanan
Affiliation:
Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India
K. M. Subbu Rathinam
Affiliation:
Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India
M. Krishnan
Affiliation:
Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India
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Abstract

The effect of photoperiod on the haemolymph protein profile in the silkworm, Bombyx mori L. larvae was investigated at photoperiod treatments of light to dark (L:D) ratios of 13:11 (control), 24:0, 18:6, 12:12, 6:18 and 0:24 hours using fifth instar larvae. The intensity of protein bands in haemolymph extracts from larvae was measured with a densitometer. The intensity was highest in the haemolymph from larvae in the 24:0 (L:D) h photoperiod. Strongly reactive antigens of SP-1 and SP-2 and weakly reactive bands of storage proteins for both male and female larvae were observed in the 24:0 (L:D) h photoperiod.

Résumé

L'effet de la photopériode sur le profil protéinique de l'haemolymphe chez la chenille du ver-à-soie, Bombyx mori L. soumise aux traitements photopériodiques de 13L:11D (témoin), 24:0, 18:6, 12:12, 6:18 et 0:24 h a été étudié au niveau des larves du 5ème instar. L'intensité des bandes de protéines dans des extraits d'haemolymphe des larves a été mesurée à l'aide d'un densimètre. La plus forte intensité a été notée au niveau de l'haemolymphe des larves à la photopériode de 24:0 (L:D). Des antigènes fortement réactifs deis bandes SP-1 et SP-2 et des bandes faiblement réactives des protéines de stockage pour les larves mâles et femelles ont été observées à la photopériode de 24:0 (L:D).

Keywords

photoperiodshaemolymphprotein profileBombyx moriphotopériodehaemolympheprofile protéiniqueBombyx mori
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 16 , Issue 2 , June 1995 , pp. 177 - 182
Copyright
Copyright © ICIPE 1995

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