Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T12:57:39.099Z Has data issue: false hasContentIssue false
  • English
  • Français

An electron microscopic study of normal haemocytes of Poecilocerus pictus (Fab.) and their response to injected yeast cells

Published online by Cambridge University Press:  19 September 2011

P. R. Sharma ,
K. Tikku  and
Bhaskar P. Saxena
P. R. Sharma
Affiliation:
Division of Insecticides and Insect Physiology, Regional Research Laboratory, Jammu Tawi-180 001, India
K. Tikku
Affiliation:
Division of Insecticides and Insect Physiology, Regional Research Laboratory, Jammu Tawi-180 001, India
Bhaskar P. Saxena
Affiliation:
Division of Insecticides and Insect Physiology, Regional Research Laboratory, Jammu Tawi-180 001, India
Get access

Abstract

Five types of haemocytes were identified in Poecilocerus pictus (Fab.) and their ultrastructural details and differential counts were studied. The types identified are the prohaemocyte, plasmatocyte, granular haemocyte, oenocytoid and coagulocyte. In response to the injection of yeast cells into the haemocoel, changes in haemocyte morphology and differential counts occurred. The predominant cell involved in phagocytosis is the plasmatocyte, followed by granular haemocyte.

Résumé

Clinque éspéces des hemocites ont été identifeiée en Poecilocerus pictus (Fab.) et leur détails ultrastructural et comptes différentielle ont été étudiés. Les espèces identifiées sont prohemocite, plasmatocite, granulocite, oenocitoid et coagulocite. Par suite d'injection de suspension cellulaire de la levure dans le haemocoel, la morphologie des ces celluss a été modifiées et les conptes différentielle ont été variées. Le rôle prédominant est joué par plasmatocites suivre par granulocites dans le processus de phagocitosis pour le mécanism de défence.

Keywords

HaemocytesultrastructurePoecilocerus pictusimmune response
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 7 , Issue 1 , February 1986 , pp. 85 - 91
Copyright
Copyright © ICIPE 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Akai, H. and Sato, S. (1971) An ultrastructural study of the hemopoietic organs of the silkworm, Bombyx mori. J. Insect Physiol. 17, 1665–1676.CrossRefGoogle Scholar
Akai, H. and Sato, S. (1973) Ultrastructure of the larval hemocytes of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). Int. J. Insect Morph. Embryol. 2, 207–231.CrossRefGoogle Scholar
Akai, H. and Sato, S. (1976) Surface ultrastructure of the larval hemocytes of the silkworm, Bombyx mori L. (Lep-idoptera: Bombycidae). Int. J. Insect Morph. Embryol. 5, 17–21.CrossRefGoogle Scholar
Akai, H. and Sato, S. (1979) Surface and internal ultrastructure of hemocytes of some insects. In Insect Hemocytes, Development, Forms, Functions and Techniques (Edited by Gupta, A. P.), pp. 129–154. Cambridge University Press, Cambridge.CrossRefGoogle Scholar
Chain, B. M. and Anderson, R. S. (1983) Inflammation in insects: The release of a plasmatocyte depletion factor following interaction between bacteria and haemocytes. J. Insect Physiol. 29, 1–4.CrossRefGoogle Scholar
Devauchelle, G. (1971) Etude ultrastructurale des hemocytes du Coléoptère Melolonlha melolontha (L.). J. Ullrastruct. Res. 34, 492–516.CrossRefGoogle ScholarPubMed
Goffinet, G. and Grégoire, Ch. (1975) Coagulocyte alterations in clotting hemolymph of Carausius morosus L. Archs int. Physiol. Biochim. 83, 707–722.Google ScholarPubMed
Gupta, A. P. (1969) Studies of the blood of Meloidae (Coleoptera). 1. The haemocytes of Epicauta cinerea (Forster), and a synonymy of haemocyte terminologies. Cytologia 34, 300–344.CrossRefGoogle Scholar
Hoffmann, J. A., Stoekel, M. E., Porte, A. and Joly, P. (1969) Ultrastructure des hemocytes de Locusta migratoria (Orthoptère). C.r. Acad. Sei., Paris 266, 503–505.Google Scholar
Hoffmann, D., Brehelin, M. and Hoffmann, J. A. (1974) Modifications of the hemogram and of the hemocytopoietic tissue of male adults of Locusta migraloria (Orthoptera) after injection of Bacillus thuringiensis. J. invertebr. Path. 24, 238–247.CrossRefGoogle Scholar
Jones, J. C. (1967) Normal differential counts of haemocytes in relation to ecdysis and feeding in Rhodnius. J. Insect Physiol. 13, 1133–1141.CrossRefGoogle Scholar
Kamionek, M. and Seryczynska, H. (1976) Changes in hemocyte ultrastructure of Galleria mellonella L. (Lep., Galeriidae) caterpillars due to the effect of fungi and parasitic nematodes. Bull. Acad. pol. Sci. Cl. II Sér. Sci. biol. 24, 483–485.Google Scholar
Nittono, Y. (1960) Studies on the blood cells in the silkworm, Bombyx mori L. Bull, sericult. exp. Stn, Tokyo 16, 171–266.Google Scholar
Raina, A. K. (1976) Ultrastructure of the larval hemocytes of the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae). Int. J. Insect Morph. Embryol. 5, 187–195.CrossRefGoogle Scholar
Ratcliffe, N. A. and Gagen, S. J. (1977) Studies on the in vivo cellular reactions of insects: An ultrastructural analysis of nodule formation in Galleria mellonella. Tissue Cell 9, 73–85.CrossRefGoogle ScholarPubMed
Seryczynska, H., Kamionek, M. and Sander, H. (1974) Defensive reaction of caterpillars of Galleria mellonella L. in relation to bacteria Achromobacter nematophilus Poinar et Thomas (Eubacteriales: Achromobacteriacae) and bacteria-free nematodes Neoaplectana carpocapsae Weiser (Nematoda: Steinernematidae). Bull. Acad. pol. Sci. Cl. II Sér. Sci. biol. 22, 193–196.Google Scholar
Takada, M. and Kitano, H. (1971) Studies on the larval hemocytes in the cabbage white butterfly, Pieris rapae crucivora Boisduval, with special reference to hemocyte classification, phagocytic activity and encapsulative capacity. Kontyu 39, 385–394.Google Scholar
Toumanoff, C. (1949) Les maladies microbienne et l'immunité naturelle chez les insectes. Revue can. Biol. 8, 343–369.Google Scholar
Wago, H. and Ichikawa, Y. (1979) Hemocytic reactions to foreign cells in the silkworm, Bombyx mori, during postembryonic development. Appl. ent. Zool. 14, 36–43.CrossRefGoogle Scholar
Wittig, G. (1966) Phagocytosis by blood cells in healthy and diseased caterpillars. II. A consideration of the method of making hemocyte counts. J. invertebr. Path. 8, 461–477.CrossRefGoogle Scholar
Yeager, J. F. (1945) The blood picture of the southern armyworm (Prodenia eridania). J. agric. Res. 71, 1–40.Google Scholar