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Amylase activity in the gut homogenate of the kola weevil, Sophrorhinus insperatus Faust and its response to inhibitors from kola nuts

Published online by Cambridge University Press:  19 September 2011

C. O. Adedire  and
R. A. Balogun
C. O. Adedire*
Affiliation:
Department of Zoology, Obafemi Awolowo University, Ile-Ife, Nigeria
R. A. Balogun
Affiliation:
Department of Zoology, Obafemi Awolowo University, Ile-Ife, Nigeria
*
* Address for correspondence: Department of Biology, Federal University of Technology, P.M.B. 704, Akure, Nigeria.
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Abstract

Some properties of the gut amylase of Sophrorhinus insperatus Faust have been studied. The enzyme has maximum activity at pH 5.0 and 45°C. It is activated by C−, Ca2+ and Cd2+ tons and inhibited by Cu2+ and Hg2+ lons. Low Michaelis constants of 0.26 mg/ml and 032 mg/ml were obtained for the larva and adult gut amylases. Enzymatic activity was found to be consistently higher in the larva than in adult stage, probably suggesting the larval stage as the most destructive stage. Extracts of Cola nitida (Vent) Schott and Endlicher and C. acuminata (Pal de Beauv) Schott and Endlicher, inhibited amylase activity in vitro at varying magnitudes. The higher level of inhibitors in C. acuminata is an index of its low susceptibility to weevil attack both on the field and at storage.

Résumé

Quelques propriétes d'amylase de I'intestin du Sophrorhinus insperatus Faust ont été étudiées. L'activité optimum de l'enzyme se presente au pH 5.0 et à la temperature de 45°C. II a été active par ces ions: Cl−, Ca2+ et Cd2+ et, on l'a fait emp^cher par ces ions: Cu2+ et Hg2+. Les petites constantes de Michaelis, obtenues pour la larve et le grand intestin amylacé étaient respectivement 0.26 mg/ml et 032 mg/ml. On a constaté que le régime d'activité de l'enzyme était beaucoup plus prononcé pour la larve qu'à l'était plus âgé.

Cet éffet nous à faire deduire que l'état de la larve est l'état le plus destructif. Les extraits de Cola nitida (Vent) Schott et Endlicher, et C. acuminata (Pal de Beauv) Schott et Endlicher empêchaient l'activité in vitro aux dégrés divers. L'éffet des empecheurs était prononcé en C. acuminata et ceci signifie qu'il est peu susceptible de l'effet du charançon au chantier et à l'emmagasinage.

Keywords

Sophrorhinus insperatusamylasekola nutsinhibitorsgut homogenateL'amylasele kolales empecheurs I'intestin
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 13 , Issue 2 , April 1992 , pp. 223 - 230
Copyright
Copyright © ICIPE 1992

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