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Spectral features of biogenic calcium carbonates and implications for astrobiology

Published online by Cambridge University Press:  10 September 2014

B. L. Berg ,
J. Ronholm ,
D. M. Applin ,
P. Mann ,
M. Izawa ,
E. A. Cloutis  and
L. G. Whyte
B. L. Berg
Affiliation:
Department of Geography, University of Winnipeg, Winnipeg, Manitoba, Canada
J. Ronholm
Affiliation:
Department of Natural Resource Sciences, McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada
D. M. Applin
Affiliation:
Department of Geography, University of Winnipeg, Winnipeg, Manitoba, Canada
P. Mann
Affiliation:
Department of Geography, University of Winnipeg, Winnipeg, Manitoba, Canada
M. Izawa
Affiliation:
Department of Geography, University of Winnipeg, Winnipeg, Manitoba, Canada
E. A. Cloutis*
Affiliation:
Department of Geography, University of Winnipeg, Winnipeg, Manitoba, Canada
L. G. Whyte
Affiliation:
Department of Natural Resource Sciences, McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada
*
e-mail: [email protected]
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Abstract

The ability to discriminate biogenic from abiogenic calcium carbonate (CaCO3) would be useful in the search for extant or extinct life, since CaCO3 can be produced by both biotic and abiotic processes on Earth. Bioprecipitated CaCO3 material was produced during the growth of heterotrophic microbial isolates on medium enriched with calcium acetate or calcium citrate. These biologically produced CaCO3, along with natural and synthetic non-biologically produced CaCO3 samples, were analysed by reflectance spectroscopy (0.35–2.5 μm), Raman spectroscopy (532 and 785 nm), and laser-induced fluorescence spectroscopy (365 and 405 nm excitation). Optimal instruments for the discrimination of biogenic from abiogenic CaCO3 were determined to be reflectance spectroscopy, and laser-induced fluorescence spectroscopy. Multiple absorption features in the visible light region occurred in reflectance spectra for most biogenic CaCO3 samples, which are likely due to organic pigments. Multiple fluorescence peaks occurred in emission spectra (405 nm excitation) of biogenic CaCO3 samples, which also are best attributed to the presence of organic compounds; however, further analyses must be performed in order to better determine the cause of these features to establish criteria for confirming the origin of a given CaCO3 sample. Raman spectroscopy was not useful for discrimination since any potential Raman peaks in spectra of biogenic carbonates collected by both the 532 and 785 nm lasers were overwhelmed by fluorescence. However, this also suggests that biogenic carbonates may be identified by the presence of this organic-associated fluorescence. No reliable spectroscopic differences in terms of parameters such as positions or widths of carbonate-associated absorption bands were found between the biogenic and abiogenic carbonate samples. These results indicate that the presence or absence of organic matter intimately associated with carbonate minerals is the only potentially useful spectral discriminator for the techniques that were examined, and that multiple spectroscopic techniques are capable of detecting the presence of associated organic materials. However, the presence or absence of intimately associated organic matter is not, in itself, an indicator of biogenicity.

Keywords

biomarkersbiomineralizationcarbonatesexobiologyMarsspectroscopy
Type
Research Article
Information
International Journal of Astrobiology , Volume 13 , Issue 4 , October 2014 , pp. 353 - 365
Copyright
Copyright © Cambridge University Press 2014 

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References

Ammor, M.S. (2007). Recent advances in the use of intrinsic fluorescence for bacterial identification and characterization. J. Fluoresc. 17, 455–459.CrossRefGoogle ScholarPubMed
Armstrong, G.A. & Hearst, J.E. (1996). Carotenoids 2: genetics and molecular biology of carotenoid pigment biosynthesis. FASEB J 10, 228–237.Google Scholar
Banfield, J.F., Moreau, J.W., Chan, C.S., Welch, S.A. & Little, B. (2001). Mineralogical biosignatures and the search for life on Mars. Astrobiology 1, 447–465.CrossRefGoogle ScholarPubMed
Barton, L.L. (2005). Structural and Functional Relationships in Prokaryotes. Springer.Google Scholar
Bibring, J.-P., et al. (2005). Mars surface diversity as revealed by the OMEGA/Mars Express observations. Science 307, 1576–1581.Google Scholar
Bischoff, W.D., Bishop, F.C. & Mackenzie, F.T. (1983). Biogenically produced magnesian calcite; inhomogeneities in chemical and physical properties; comparison with synthetic phases. Am. Mineral. 68, 1183–1188.Google Scholar
Bischoff, W.D., Sharma, S.K. & Mackenzie, F.T. (1985). Carbonate ion disorder in synthetic and biogenic magnesian calcites; a Raman spectral study. Am. Mineral. 70, 581–589.Google Scholar
Blanco, A., Orofino, V., D'Elia, M., Fonti, S., Mastandrea, A., Guido, A. & Russo, F. (2013). Infrared spectroscopy of microbially induced carbonates and past life on Mars. Icarus 226, 119–126.Google Scholar
Boquet, E., Boronat, A. & Ramos-Cormenzana, A. (1973). Production of calcite (calcium carbonate) crystals by soil bacteria is a general phenomenon. Nature 246, 527–529.Google Scholar
Boynton, W.V., Ming, D.W., Kounaves, S.P., Young, S.M.M., Arvidson, R.E., Hecht, M. H. et al. (2009). Evidence for calcium carbonate at the Mars Phoenix landing site. Science 325, 61–64.Google Scholar
Bozlee, B.J., Misra, A.K., Sharma, S.K. & Ingram, M. (2005). Remote Raman and fluorescence studies of mineral samples. Spectrochim. Acta A, Mol. Biomol. Spectrosc. 61, 2342–2348.Google Scholar
Clark, R.N., King, T.V., Klejwa, M., Swayze, G.A. & Vergo, N. (1990). High spectral resolution reflectance spectroscopy of minerals. J. Geophys. Res. Solid Earth (1978–2012) 95, 12653–12680.Google Scholar
Cockell, C.S., Catling, D.C., Davis, W.L., Snook, K., Kepner, R.L., Lee, P. & McKay, C.P. (2000). The ultraviolet environment of Mars: biological implications past, present, and future. Icarus 146, 343–359.Google Scholar
Davis, W.L. & McKay, C.P. (1996). Origins of life: a comparison of theories and application to Mars. Orig. Life Evol. Biosph. 26, 61–73.Google Scholar
De Angelis, S., De Sanctis, M.C., Ammannito, E., Altieri, F., Carli, C., Frigeri, A., Boccaccini, A. & Giardino, M. (2014). Analysis of rocks particulates by VNIR spectroscopy with MA_Miss instrument breadboard. Lunar and Planetary Science Conference, 45, abstract #1713.Google Scholar
Delaye, L. & Lazcano, A. (2005). Prebiological evolution and the physics of the origin of life. Phys. Life Rev. 2, 47–64.Google Scholar
Dieser, M., Greenwood, M. & Foreman, C.M. (2010). Carotenoid pigmentation in Antarctic heterotrophic bacteria as a strategy to withstand environmental stresses. Arct. Antarct. Alpine Res. 42, 396–405.Google Scholar
Dworkin, M. & Falkow, S. (2006). The Prokaryotes: Vol. 7: Proteobacteria: Delta and Epsilon Subclasses. Deeply Rooting Bacteria. Springer.Google Scholar
Edwards, H.G.M. (2010). Raman spectroscopic approach to analytical astrobiology: the detection of key geological and biomolecular markers in the search for life. Philos. Trans. Royal Soc. A 368, 3059–3065.Google Scholar
Ehlmann, B.L., Mustard, J.F., Murchie, S.L., Poulet, F., Bishop, J.L., Brown, A.J., et al. (2008). Orbital identification of carbonate-bearing rocks on Mars. Science 322, 1828–1832.Google Scholar
Ehrlich, H.L. (2002). Geomicrobiology, 4th edn. Marcel Dekker.CrossRefGoogle Scholar
Ellery, A., Kolb, C., Lammer, H., Parnell, J., Edwards, H., Richter, L., Patel, M., Romstedt, J., Dickensheets, D., Steele, A. & Cockell, C. (2002). Astrobiological instrumentation for Mars – the only way is down. Int. J. Astrobiol. 1, 365–380.Google Scholar
Gaffey, S.J. (1987). Spectral reflectance of carbonate minerals in the visible and near infrared (0.35–2.55 microns): calcite, aragonite, and dolomite. Am. Mineral. 71, 151–162.Google Scholar
Holm, N.G. & Andersson, E. (2005). Hydrothermal simulation experiments as a tool for studies of the origin of life on earth and other terrestrial planets: a review. Astrobiology 5, 444–460.Google Scholar
Izawa, M.R.M., Applin, D.M., Norman, L. & Cloutis, E.A. (2014). Reflectance spectroscopy (350–2500 nm) of solid-state polycyclic aromatic hydrocarbons (PAHs). Icarus 237, 159–181.Google Scholar
Korablev, O., et al. (2013). AOTF near-IR spectrometers for study of lunar and martian surface composition. European Planetary Science Congress 8, abstract #EPSC2013-50-1.Google Scholar
Mann, S. (2001). Biomineralization: Principles and Concepts in Bioinorganic Materials Chemistry. Oxford University Press.Google Scholar
Marshall, C.P. & Marshall, A.O. (2010). The potential of Raman spectroscopy for the analysis of diagenetically transformed carotenoids. Philos. Trans. Royal Soc. A Math. Phys. Eng. Sci. 368, 3137–3144.Google Scholar
Michalski, J.R. & Niles, P.B. (2010). Deep crustal carbonate rocks exposed by meteor impact on Mars. Nat. Geosci. 3, 751–755.Google Scholar
McKay, C.P. (1997). The search for life on Mars. Orig. Life Evol. Biosp. 27, 263–289.Google Scholar
McKay, D.S.D., Gibson, E.K.E., Thomas-Keprta, K.L.K., Vali, H.H., Romanek, C.S.C., Clemett, S.J.S., Chillier, X.D.X., Maechling, C.R.C. & Zare, R.N.R. (1996). Search for past life on Mars: possible relic biogenic activity in martian meteorite ALH84001. Science 273, 924–930.Google Scholar
Moore, M.M., Breedveld, M.W. & Autor, A.P. (1989). The role of carotenoids in preventing oxidative damage in the pigmented yeast, Rhodotorula mucilaginosa . Arch. Biochem. Biophys. 270, 419–431.CrossRefGoogle ScholarPubMed
Morris, R.V., Ruff, S.W., Gellert, R., Ming, D.W., Arvidson, R.E., Clark, B.C., et al. (2010). Identification of carbonate-rich outcrops on Mars by the Spirit rover. Science 329, 421–424.Google Scholar
Murchie, S., et al. (2007). Compact reconnaissance imaging spectrometer for Mars (CRISM) on Mars Reconnaissance Orbiter (MRO). J. Geophys. Res. 112, E05S03. DOI: 10.1029/2006JE002682.Google Scholar
Nassau, K. (1978). The origins of color in minerals. Am. Mineral. 63, 219–229.Google Scholar
Orofino, V., Blanco, A., D'Elia, M., Licchelli, D. & Fonti, S. (2007). Infrared transmission spectroscopy of carbonate samples of biotic origin relevant to Mars exobiological studies. Icarus 187, 457–463.Google Scholar
Orofino, V., Blanco, A., D'Elia, M., Fonti, S. & Licchelli, D. (2009). Time-dependent degradation of biotic carbonates and the search for past life on Mars. Planet. Space Sci. 57, 632–639.Google Scholar
Pilorget, C., Bibring, J.-P. & the MicrOmega Team (2012). The MicrOmega instrument onboard ExoMars and future missions: an IR hyperspectral microscope to analyze samples at the grain scale and characterize early Mars processes. Third Conference on Early Mars, abstract #7006.Google Scholar
Raulin, F. & McKay, C.P. (2002). The search for extraterrestrial life and prebiotic chemistry. Planet. Space Sci. 50, 655.Google Scholar
Rivadeneyra, M.A., Párraga, J., Delgado, R., Ramos-Cormenzana, A. & Delgado, G. (2004). Biomineralization of carbonates by Halobacillus trueperi in solid and liquid media with different salinities. FEMS Microbiol. Ecol. 48, 39–46.Google Scholar
Sánchez-Román, M., Romanek, C.S., Fernández-Remolar, D.C., Sánchez-Navas, A., McKenzie, J.A., Pibernat, R.A. & Vasconcelos, C. (2011). Aerobic biomineralization of Mg-rich carbonates: implications for natural environments. Chem. Geol. 281, 143–150.CrossRefGoogle Scholar
Schulman, J.H. & Compton, W.D. (1962). Color Centers in Solids, vol. 2. Pergamon.Google Scholar
Simoneit, B.R.T. (2004). Prebiotic organic synthesis under hydrothermal conditions: an overview. Adv. Space Res. 33, 88–94.Google Scholar
Stalport, F., et al. (2005). Search for past life on Mars: physical and chemical characterization of minerals of biotic and abiotic origin: part 1 – Calcite. Geophys. Res. Lett. 32, L23205.Google Scholar
Stalport, F., Person, A., Cabane, M., Ausset, P., Coll, P., Szopa, C. & Navarro-Gonzalez, R. (2008). Biominerals on Mars: the potential for carbonates to be life indicators. In 37th COSPAR Scientific Assembly, 37, p. 3017.Google Scholar
Steven, B., Briggs, G., McKay, C.P., Pollard, W.H., Greer, C.W. & Whyte, L.G. (2007). Characterization of the microbial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods. FEMS Microbiol. Ecol. 59, 513–523.Google Scholar
Stopar, J.D., Lucey, P.G., Sharma, S.K., Misra, A.K., Taylor, G.J. & Hubble, H.W. (2005). Raman efficiencies of natural rocks and minerals: performance of a remote Raman system for planetary exploration at a distance of 10 meters. Spectrochim. Acta A, Mol. Biomol. Spectrosc. 61, 2315–2323.Google Scholar
Storrie-Lombardi, M.C. & Sattler, B. (2009). Laser-induced fluorescence emission (L.I.F.E.): in situ nondestructive detection of microbial life in the ice covers of Antarctic lakes. Astrobiology 9, 659–672.Google Scholar
Suo, Z., Avci, R., Schweitzer, M.H. & Deliorman, M. (2007). Porphyrin as an ideal biomarker in the search for extraterrestrial life. Astrobiology 7, 605–615.Google Scholar
Thompson, S.P., Parker, J.E. & Tang, C.C. (2014). Thermal breakdown of calcium carbonate and constraints on its use as a biomarker. Icarus 229, 1–10.Google Scholar
Thöny-Meyer, L. (1997). Biogenesis of respiratory cytochromes in bacteria. Microbiol. Mol. Biol. Rev. 61, 337–376.Google ScholarPubMed
Tucker, E.M. & Wright, V.P. (1990). Carbonate Sedimentology. Oxford University Press, pp. 482.Google Scholar
Urmos, J., Sharma, S.K. & Mackenzie, F.T. (1991). Characterization of some biogenic carbonates with Raman spectroscopy. Am. Mineral. 76, 641–646.Google Scholar
Vecht, A. & Ireland, T.G. (2000). The role of vaterite and aragonite in the formation of pseudo-biogenic carbonate structures: implications for Martian exobiology. Geochim. Cosmochim. Acta 64, 2719–2725.Google Scholar
Veizer, J. (1983). Trace elements and isotopes in sedimentary carbonates. Rev. Mineral. Geochem. 11, 265–299.Google Scholar
Villar, S.E.J. & Edwards, H.G. (2006). Raman spectroscopy in astrobiology. Anal. Bioanal. Chem. 384, 100–113.Google Scholar
Wang, J. & Mullins, O.C. (1997). Fluorescence of limestones and limestone components. Appl. Spectrosc. 51, 1890–1895.Google Scholar
White, A.F. (1975). Sodium and potassium coprecipitation in calcium carbonate. PhD Thesis, Northwestern University.Google Scholar
White, S.N. (2009). Laser Raman spectroscopy as a technique for identification of seafloor hydrothermal and cold seep minerals. Chem. Geol. 259, 240–252.Google Scholar
Workman, J. Jr. & Weyer, L. (2012). Practical Guide and Spectral Atlas for Interpretive Near-Infrared Spectroscopy. CRC Press.Google Scholar