Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-24T22:47:36.680Z Has data issue: false hasContentIssue false
  • English
  • Français

Rapid Monitoring by Quantitative Polymerase Chain Reaction for Pathogenic Aspergillus During Carpet Removal From a Hospital

Published online by Cambridge University Press:  02 January 2015

Alice N. Neely ,
Vince Gallardo ,
Ed Barth ,
Richard A. Haugland ,
Glenn D. Warden  and
Stephen J. Vesper
Alice N. Neely*
Affiliation:
Shriners Burns Hospital and the Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio
Vince Gallardo
Affiliation:
National Risk Management Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio
Ed Barth
Affiliation:
National Risk Management Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio
Richard A. Haugland
Affiliation:
National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio
Glenn D. Warden
Affiliation:
Shriners Burns Hospital and the Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio
Stephen J. Vesper
Affiliation:
National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio
*
Shriners Burns Hospital, 3229 Burnet Avenue, Cincinnati, OH 45229-3095
Get access Rights & Permissions [Opens in a new window]

Abstract

Monitoring for pathogenic Aspergillus species using a rapid, highly sensitive, quantitative polymerase chain reaction technique during carpet removal in a burn unit provided data that allowed patients to be safely returned to the refloored area sooner than if only conventional culture monitoring had been used.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 25 , Issue 4 , April 2004 , pp. 350 - 352
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Schwacha, MG. Macrophages and post-burn immune dysfunction. Burns 2003;29:1–14.Google Scholar
2.Bartley, JM. APIC state-of-the-art report: the role of infection control during construction in health care facilities. Am J Infect Control 2000;28:156–169.Google Scholar
3.Gerson, SL, Parker, P, Jacobs, MR, et al.Aspergillosis due to carpet contamination. Infect Control Hosp Epidemiol 1994;15:221–223.CrossRefGoogle ScholarPubMed
4.Thio, CL, Smith, D, Merz, WG, et al.Refinements of environmental assessment during an outbreak investigation of invasive aspergillosis in leukemia and bone marrow unit. Infect Control Hosp Epidemiol 2000;21:18–23.Google Scholar
5.Haugland, RA, Vesper, SJ. Identification and Quantification of Specific Fungi and Bacteria. US patent 6,387,652. May 14, 2002.Google Scholar
6.SKC. Button Aerosol Sampler: An Efficient Inhalable Sampler. Eighty Four, PA: SKC; 2004. Available at www.skcinc.com/prod/225-360.asp. Accessed January 30, 2004.Google Scholar
7.Sigler, L, Verweij, PE. Aspergillus, Fusarium, and other opportunistic moniliaceous fungi. In: Murray, PR, Baron, EJ, Jorgensen, JH, Pfaller, MA, Yolken, RH, eds. Manual of Clinical Microbiology, ed. 8. Washington, DC: American Society of Microbiology Press; 2003:1736.Google Scholar
8.Haugland, RA, Brinkman, N, Vesper, SJ. Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis. J Microbiol Methods 2002;50:319–323.Google Scholar
9.Brinkman, NE, Haugland, RA, Wymer, LJ, et al.Evaluation of a rapid, quantitative real-time PCR method for cellular enumeration of pathogenic Candida species in water. Appl Environ Microbiol 2003;69:1775–1783.Google Scholar
10.Haugland, RA, Varma, M, Wymer, LJ, Vesper, SJ. Quantitative PCR analysis of some Aspergillus, Penicillium and Paecilomyces species. Syst Appl Microb 2004. In press.Google Scholar
11.Neely, AN, Orloff, MM. Survival of some medically important fungi in hospital fabrics and plastics. J Clin Microbiol 2001;39:3360–3361.CrossRefGoogle ScholarPubMed