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Polymerase Chain Reaction Identification of Coagulase-Negative Staphylococci and of Strain Diversity and Spread of Staphylococcus epidermidis in a Major Medical Center in Lebanon

Published online by Cambridge University Press:  21 June 2016

Inaya M. Abdallah ,
George E Araj ,
Ghassan M. Matar ,
George Abdelnour ,
Marwan Uwaydah  and
Alexander M. Abdelnoor
Inaya M. Abdallah
Affiliation:
Department of Microbiology and Immunology, American University of Beirut Medical Center, Lebanon
George E Araj
Affiliation:
Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Lebanon
Ghassan M. Matar
Affiliation:
Department of Microbiology and Immunology, American University of Beirut Medical Center, Lebanon
George Abdelnour
Affiliation:
Hôpital Notre Dame Des Secours, Byblos, Lebanon
Marwan Uwaydah
Affiliation:
Department of Microbiology and Immunology, American University of Beirut Medical Center, Lebanon
Alexander M. Abdelnoor*
Affiliation:
Department of Microbiology and Immunology, American University of Beirut Medical Center, Lebanon
*
Department of Microbiology and Immunology, American University of Beirut, PO Box 11-0236, Riad El-Solh, Beirut 11072020, Lebanon ([email protected])
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Abstract

A 2-step polymerase chain reaction (PCR) assay and random amplification of polymorphic DNA (RAPD) analysis, respectively, were assessed to identify coagulase-negative staphylococci organisms to the species level and to determine the strain diversity and spread of Staphylococcus epidermidis, the most frequently isolated species, in a medical center in Beirut, Lebanon. Our data indicated that PCR was faster and was more efficient in identifying S. epidermidis isolates than is conventional biochemical testing. RAPD analysis have shown that S. epidermidis strains were scattered across the different clinical services, demonstrating various clusters of infection in the medical center.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 7 , July 2006 , pp. 781 - 783
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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