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Molecular Typing of Mycobacterium chelonae Isolates From a Pseudo-outbreak Involving an Automated Bronchoscope Washer

Published online by Cambridge University Press:  02 January 2015

Alexandra Chroneou ,
Sarah K. Zimmerman ,
Steven Cook ,
Sandra Willey ,
Jane Eyre-Kelly ,
Nikolaos Zias ,
Daniel S. Shapiro ,
John F. Beamis  and
Donald E. Craven
Alexandra Chroneou
Affiliation:
Departments of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington
Sarah K. Zimmerman
Affiliation:
Departments of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington Department of Microbiology and Laboratory Medicine, Lahey Clinic Medical Center, Burlington
Steven Cook
Affiliation:
Department of Microbiology and Laboratory Medicine, Lahey Clinic Medical Center, Burlington
Sandra Willey
Affiliation:
Infection Control and Hospital Epidemiology, Lahey Clinic Medical Center, Burlington
Jane Eyre-Kelly
Affiliation:
Infection Control and Hospital Epidemiology, Lahey Clinic Medical Center, Burlington
Nikolaos Zias
Affiliation:
Departments of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington
Daniel S. Shapiro
Affiliation:
Infectious Diseases, Lahey Clinic Medical Center, Burlington Department of Microbiology and Laboratory Medicine, Lahey Clinic Medical Center, Burlington Boston University School of Medicine, Boston, Massachusetts
John F. Beamis
Affiliation:
Departments of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington Tufts University School of Medicine, Boston, Massachusetts
Donald E. Craven*
Affiliation:
Infectious Diseases, Lahey Clinic Medical Center, Burlington Tufts University School of Medicine, Boston, Massachusetts
*
Department of Infectious Diseases, Lahey Clinic Medical Center, 41 Mall Rd., Burlington, MA 01805 ([email protected])
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Abstract

We describe a pseudo-outbreak of Mycobacterium chelonae infection in bronchoalveolar lavage fluid from 9 patients that was traced to contamination of an automated bronchoscope washer. Molecular typing using repetitive extragenic palindromic polymerase chain reaction was helpful in confirming epidemiologic and clinical findings.

Type
Concise Communication
Information
Infection Control & Hospital Epidemiology , Volume 29 , Issue 11 , November 2008 , pp. 1088 - 1090
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2008

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References

1.Wallace, RJ Jr, Brown, BA, Griffith, DE. Nosocomial outbreaks/pseudo-outbreaks caused by nontuberculous mycobacteria. Annu Rev Microbiol 1998;52:453–490.CrossRefGoogle ScholarPubMed
2.Healy, M, Huong, J, Bittner, T, et al.Microbial DNA typing by automated repetitive-sequence-based PCR. J Clin Microbiol 2005;43:199–207.CrossRefGoogle ScholarPubMed
3.van Embden, JD, Cave, MD, Crawford, JT, et al.Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 1993;31:406–409.CrossRefGoogle ScholarPubMed
4.Griffith, DE, Aksamit, T, Brown-Elliott, BA, et al: an official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 2007;175:367–416.CrossRefGoogle Scholar
5.Falkinham, JO, 3rd. Epidemiology of infection by nontuberculous mycobacteria. Clin Microbiol Rev 1996;9:177–215.CrossRefGoogle ScholarPubMed
6.Hussain, SA. Fiberoptic bronchoscope-related outbreak of infection with Pseudomonas. Chest 1978;74:483.CrossRefGoogle ScholarPubMed
7.Martone, WJ, Osterman, CA, Fisher, KA, Wenzel, RRPseudomonas cepacia: implications and control of epidemic nosocomial colonization. Rev Infect Dis 1981;3:708–715.CrossRefGoogle ScholarPubMed
8.Wheeler, PW, Lancaster, D, Kaiser, AB. Bronchopulmonary cross-colonization and infection related to mycobacterial contamination of suction valves of bronchoscopes. J Infect Dis 1989;159:954–958.CrossRefGoogle ScholarPubMed
9.Weinstein, RA, Stamm, WE. Pseudoepidemics in hospital. Lancet 1977;2:862–864.CrossRefGoogle ScholarPubMed
10.Gubler, JG, Salfinger, M, von Graevenitz, A. Pseudoepidemic of nontuberculous mycobacteria due to a contaminated bronchoscope cleaning machine: report of an outbreak and review of the literature. Chest 1992;101:1245–1249.CrossRefGoogle ScholarPubMed