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Molecular Epidemiology of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Isolated From Environmental and Clinical Specimens in a Cardiac Surgery Intensive Care Unit

Published online by Cambridge University Press:  02 January 2015

Guillaume Kac ,
Isabelle Podglajen ,
Sabine Vaupré ,
Nathalie Colardelle ,
Annie Buu-Hoï  and
Laurent Gutmann
Guillaume Kac*
Affiliation:
Unité D'Hygiène Hospitalière, Hôpital Broussais, Paris, France
Isabelle Podglajen
Affiliation:
Laboratoire de Microbiologie, Hôpital Broussais, Paris, France
Sabine Vaupré
Affiliation:
Unité D'Hygiène Hospitalière, Hôpital Broussais, Paris, France
Nathalie Colardelle
Affiliation:
Laboratoire de Microbiologie, Hôpital Broussais, Paris, France
Annie Buu-Hoï
Affiliation:
Laboratoire de Microbiologie, Hôpital Broussais, Paris, France
Laurent Gutmann
Affiliation:
Laboratoire de Microbiologie, Hôpital Broussais, Paris, France
*
Unité D'Hygiène Hospitalière, Hôpital Européen Georges Pompidou, 20-40 rue Leblanc, 75908 Paris cedex 15, France
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Abstract

Objectives:

To investigate environmental contamination by extended-spectrum beta-lactamase-producing Enterobacteriaceae and to perform a comparative molecular analysis of clinical and environmental strains.

Setting:

A 17-bed cardiac surgery intensive care unit of a 480-bed university teaching hospital.

Methods:

Following an outbreak of extended-spectrum beta-lactamase-producing Enterobacteriaceae, an environmental survey revealed extensive contamination of the environment (particularly faucets, sink drains, and the joints of the countertops) by extended-spectrum beta-lactamase-producing Enterobacteriaceae. Environmental strains were compared with clinical strains by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA.

Results:

A total of 62 environmental strains belonging to 4 species of extended-spectrum beta-lactamase-producing Enterobacteriaceae were analyzed and compared with 43 clinical strains obtained from 34 patients. Comparative molecular analysis revealed 4 identical or closely related patterns (3 from Klebsiella oxytoca and 1 from Enterobacter cloacae) between environmental and clinical strains.

Conclusions:

Moist surfaces may serve as sources of multiply resistant Enterobacteriaceae in the intensive care unit. Identification and disinfection of such sources may therefore be helpful in prevention and control of outbreaks.

Type
Orginal Articles
Information
Infection Control & Hospital Epidemiology , Volume 25 , Issue 10 , October 2004 , pp. 852 - 855
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2004 

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