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Molecular Characterization of Streptococcus Pyogenes Isolates to investigate an outbreak of Puerperal Sepsis

Published online by Cambridge University Press:  21 June 2016

Josette Raymond ,
Laurent Schlegel ,
Fabien Garnier  and
Anne Bouvet
Josette Raymond
Affiliation:
Microbiology Department, Hòpital Saint Vincent de Paul, Paris V University, Paris, France
Laurent Schlegel
Affiliation:
National Reference Center for Streptococci, Hòtel Dieu AP-HP, Paris V University, Paris, France
Fabien Garnier
Affiliation:
Microbiology Department, Hòpital Saint Vincent de Paul, Paris V University, Paris, France
Anne Bouvet*
Affiliation:
National Reference Center for Streptococci, Hòtel Dieu AP-HP, Paris V University, Paris, France
*
Centre National de Réference des Streptocoques, Service de Microbiologie, Hòtel Dieu, Université Paris V, 1 place du Parvis Notre-Dame, F 75181 Paris 04, France[email protected]
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Abstract

Objective:

To describe microbiological characteristics and epidemiologic features of an outbreak of postpartum endometritis.

Methods:

Various markers were investigated in five patients and three throat carriage isolates of Streptococcus pyogenes obtained during an outbreak of endometritis occurring in a 13-week period. Molecular characterization included biotyping, T-serotyping, emm gene sequence and restriction, pulsed-field gel electrophoresis (PFGE), and random amplified polymorphic DNA (RAPD) analysis.

Results:

Biotype, T-serotype, and genotypic data (emm analysis, PFGE, and RAPD analysis) revealed a close relationship among the isolates from three patients, suggesting that cross-contamination had occurred. These isolates were biotype 1, T type 28, and emm type 28. The isolates from one patient and one carrier differed from those of the index patient by minor variations of the emm amplicon restriction pattern, PFGE pattern, or RAPD pattern. The remaining isolates were phenotypically and genetically different.

Conclusion:

Identification of different isolates demonstrated that different strains may circulate simultaneously during a true outbreak and that the predominant strain might persist for several months.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 26 , Issue 5 , May 2005 , pp. 455 - 461
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2005

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