Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-16T21:14:12.941Z Has data issue: false hasContentIssue false
  • English
  • Français

Interinstitutional and Intrainstitutional Transmission of a Strain of Acinetobacter baumannii Detected by Molecular Analysis Comparison of Pulsed-Field Gel Electrophoresis and Repetitive Sequence–Based Polymerase Chain Reaction

Published online by Cambridge University Press:  21 June 2016

S. Saeed ,
M. G. Fakih ,
K. Riederer ,
A. R. Shah  and
R. Khatib
S. Saeed
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
M. G. Fakih
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
K. Riederer
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
A. R. Shah
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
R. Khatib*
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
*
Medical Education, St. John Hospital & Medical Center, 22101 Moross Road, Detroit, MI 48236, ([email protected])
Get access Rights & Permissions [Opens in a new window]

Abstract

Pulsed-field gel electrophoresis and repetitive sequence-based polymerase chain reaction provided comparable strain discrimination with minor discordance in typing Acinetobacter baumannii clinical isolates from patients at our hospital and affiliated institutions. Typing revealed a cluster strain with intrainstitutional and interinstitutional spread during the study period. A long-term acute care facility may have been the reservoir.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 9 , September 2006 , pp. 981 - 983
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Bergogne-Berezin, E, Towner, KJ. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 1996; 9:148–165.Google Scholar
2.Corbella, X, Montero, A, Pujol, M, et al. Emergence and rapid spread of carbapenem resistance during a large and sustained hospital outbreak of multiresistant Acinetobacter baumannii. J Clin Microbiol 2000; 38:4086–4095.CrossRefGoogle ScholarPubMed
3.Spence, RP, van der Reijden, TJ, Dijkshoorn, L, Towner, KJ. Comparison of Acinetobacter baumannii isolates from United Kingdom hospitals with predominant Northern European genotypes by amplified-fragment length polymorphism analysis. J Clin Microbiol 2004; 42:832–834.Google Scholar
4.Wisplinghoff, H, Edmond, MB, Pfaller, MA, Jones, RN, Wenzel, RP, Seifert, H. Nosocomial bloodstream infections caused by Acinetobacter species in United States hospitals: clinical features, molecular epidemiology, and antimicrobial susceptibility. Clin Infect Dis 2000; 31:690–697.CrossRefGoogle ScholarPubMed
5.Biendo, M, Laurans, G, Lefebvre, JF, Daoudi, F, Eb, F. Epidemiological study of an Acinetobacter baumannii outbreak by using a combination of antibiotyping and ribotyping. J Clin Microbiol 1999; 37:2170–2175.CrossRefGoogle ScholarPubMed
6.Quale, J, Bratu, S, Landman, D, Heddurshetti, R. Molecular epidemiology and mechanisms of carbapenems resistance in Acinetobacter baumannii endemic in New York City. Clin Infect Dis 2003; 37:214–220.Google Scholar
7.Herruzo, R, de la Cruz, J, Fernandez-Acenero, MJ, Garcia-Caballero, J. Two consecutive outbreaks of Acinetobacter baumannii 1-A in a burn intensive care unit for adults. Burns 2004; 30:419–423.Google Scholar
8.Poirel, L, Menuteau, O, Agoli, N, Cattoen, C, Nordmann, P. Outbreak of extended-spectrum beta-lactamase VEB-1-producing isolates of Acinetobacter baumannii in a French hospital. J Clin Microbiol 2003; 41:3542–3547.Google Scholar
9.Villegas, MV, Hartstein, AI. Acinetobacter outbreaks, 1977-2000. Infect Control Hosp Epidemiol 2003; 24:284–295.Google Scholar
10.Manikal, VM, Landman, D, Saurina, G, Oydna, E, Lai, H, Quale, J. Endemic carbapenems-resistant Acinetobacter species in Brooklyn, New York: city-wide prevalence, interinstitutional spread, and relation to antibiotic usage. Clin Infect Dis 2000; 31:101–106.Google Scholar