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Inactivation of Human Immunodeficiency Virus by Betadine

Published online by Cambridge University Press:  02 January 2015

Joan C. Kaplan ,
David C. Crawford ,
Amy G. Durno  and
Robert T. Schooley
Joan C. Kaplan*
Affiliation:
Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts
David C. Crawford
Affiliation:
Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts
Amy G. Durno
Affiliation:
Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts
Robert T. Schooley
Affiliation:
Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts
*
Infectious Disease Unit, Massachusetts General Hospital, Boston, MA 02114
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Abstract

Human immunodeficiency virus (HIV), the etiological agent of the acquired immunodeficiency syndrome (AIDS), was treated with either Betadine® (povidone-iodine) Solution or Betadine Surgical Scrub®. HIV inactivation was analyzed using the viral reverse transcriptase assay or by observing the cytopathic effect produced in HIV-infected, H-9, T-cell cultures. The minimum effective Betadine dose was 0.25% for complete inactivation of HIV that was treated for various time intervals (immediate vortex to ten minutes). The titer of HIV stocks used in these experiments (105 TCID50 per mL) was greater than amounts generally detected in clinical specimens. Our results provide a rationale for the use of povidone-iodine as a topical antiseptic against HIV in the clinic or laboratory.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 8 , Issue 10 , October 1987 , pp. 412 - 414
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1987

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