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Epidemiology of Methicillin-Susceptible Staphylococcus aureus in a Neonatology Ward

Published online by Cambridge University Press:  20 August 2015

Yvonne Achermann ,
Kati Seidl ,
Stefan P. Kuster ,
Nadja Leimer ,
Nina Durisch ,
Evelyne Ajdler-Schäffler ,
Stephan Karrer ,
Gabriela Senn ,
Anne Holzmann-Bürgel  and
Aline Wolfensberger
...Show all authors
Yvonne Achermann
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Kati Seidl
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Stefan P. Kuster
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Nadja Leimer
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Nina Durisch
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Evelyne Ajdler-Schäffler
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Stephan Karrer
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Gabriela Senn
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Anne Holzmann-Bürgel
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Aline Wolfensberger
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Antonio Leone
Affiliation:
Division of Neonatology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Romaine Arlettaz
Affiliation:
Division of Neonatology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Annelies S. Zinkernagel
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
Hugo Sax*
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland
*
Address correspondence to Hugo Sax, MD, University Hospital of Zurich, University of Zurich, Division of Infectious Diseases and Hospital Epidemiology, Raemistrasse 100, CH-8091 Zurich, Switzerland ([email protected]).
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Abstract

OBJECTIVE

In-hospital transmission of methicillin-susceptible Staphylococcus aureus (MSSA) among neonates remains enigmatic. We describe the epidemiology of MSSA colonization and infection in a 30-bed neonatal ward.

DESIGN

Multimodal outbreak investigation

SETTING

A public 800-bed tertiary care university hospital in Switzerland

METHODS

Investigations in 2012–2013, triggered by a MSSA infection cluster, included prospective MSSA infection surveillance, microbiologic screening of neonates and environment, onsite observations, and a prospective cohort study. MSSA isolates were characterized by pulsed-field gel electrophoresis (PFGE) and selected isolates were examined for multilocus sequence type (MLST) and virulence factors.

RESULTS

Among 726 in 2012, 30 (4.1%) patients suffered from MSSA infections including 8 (1.1%) with bacteremia. Among 655 admissions in 2013, 13 (2.0%) suffered from MSSA infections including 2 (0.3%) with bacteremia. Among 177 neonates screened for S. aureus carriage, overall 77 (44%) tested positive. A predominant PFGE-1-ST30 strain was identified in 6 of 30 infected neonates (20%) and 30 of 77 colonized neonates (39%). This persistent clone was pvl-negative, tst-positive and belonged to agr group III. We found no environmental point source. MSSA carriage was associated with central vascular catheter use but not with a particular midwife, nurse, physician, or isolette. Observed healthcare worker behavior may have propagated transmission via hands and fomites. Despite multimodal interventions, clonal transmission and colonization continued and another clone, PFGE-6-ST5, became predominant.

CONCLUSIONS

Hospital-acquired MSSA clones represent a high proportion of MSSA colonization but not MSSA infections in neonate inpatients. In contrast to persisting MSSA, transmission infection rates decreased concurrently with interventions. It remains to be established whether eradication of hospital-acquired MSSA strains would reduce infection rates further.

Infect. Control Hosp. Epidemiol. 2015;36(11):1305–1312

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 36 , Supplement 11 , November 2015 , pp. 1305 - 1312
Copyright
© 2015 by The Society for Healthcare Epidemiology of America. All rights reserved 

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Footnotes

a

Authors with equal contribution.

b

Authors with equal contribution.

PREVIOUS PRESENTATION: Parts of this study were presented as an oral presentation at the Interscience Conference on Antimicrobial Agents and Chemotherapy, San Francisco, California, USA, September 17–23, 2012.

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