Hostname: page-component-745bb68f8f-lrblm Total loading time: 0 Render date: 2025-01-08T10:29:21.839Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of qacA/B in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus from a Regional Healthcare Network in the Eastern United States

Published online by Cambridge University Press:  02 January 2015

Patrick McGann ,
Yoon I. Kwak ,
Amy Summers ,
James F. Cummings ,
Paige E. Waterman  and
Emil P. Lesho
Patrick McGann*
Affiliation:
Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland
Yoon I. Kwak
Affiliation:
Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland
Amy Summers
Affiliation:
Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland
James F. Cummings
Affiliation:
Department of Regulated Activities, Walter Reed Army Institute of Research, Silver Spring, Maryland
Paige E. Waterman
Affiliation:
Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland
Emil P. Lesho
Affiliation:
Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland
*
Walter Reed Army Institute of Research, 503 Robert Grant Avenue, 1E06, Silver Spring, MD 20910 ([email protected])
Get access Rights & Permissions [Opens in a new window]

Abstract

We describe the clinical, microbiologic, and molecular features of the first series of qacA/B-containing strains of methicillin-resistant Staphylococcus aureus from infected US patients. All qac-carrying strains were clonally diverse, and qacA strains exhibited increased tolerance to chlorhexidine as measured by minimum inhibitory concentrations, minimum bactericidal concentrations, and postexposure colony counts.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 32 , Issue 11 , November 2011 , pp. 1116 - 1119
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Smith, K, Gemmell, CG, Hunter, IS. The association between biocide tolerance and the presence or absence of qac genes among hospital-acquired and community-acquired MRSA isolates. J Antimicrob Chemother 2008;61:78–84.CrossRefGoogle ScholarPubMed
2.Batra, R, Cooper, BS, Whiteley, C, Patel, AK, Wyncoll, D, Edgeworth, JD. Efficacy and limitation of a chlorhexidine-based decolonization strategy in preventing transmission of methicillin-resistant Staphylococcus aureus in an intensive care unit. Clin Infect Dis 2010;50:210–217.CrossRefGoogle Scholar
3.DeMarco, CE, Cushing, LA, Frempong-Manso, E, Seo, SM, Jaravaza, TA, Kaatz, GW. Efflux-related resistance to norfloxacin, dyes, and biocides in bloodstream isolates of Staphylococcus aureus. Antimicrob Agents Chemother 2007;51:3235–3239.CrossRefGoogle ScholarPubMed
4.Mayer, S, Boos, M, Beyer, A, Fluit, AC, Schmitz, FJ. Distribution of the antiseptic resistance genes qacA, qacB and qacC in 497 methicillin-resistant and -susceptible European isolates of Staphylococcus aureus. J Antimicrob Chemother 2001;47:896–897.Google Scholar
5.Noguchi, N, Hase, M, Kitta, M, Sasatsu, M, Deguchi, K, Kono, M. Antiseptic susceptibility and distribution of antiseptic-resistance genes in methicillin-resistant Staphylococcus aureus. FEMS Microbiol Lett 1999;172:247–253.CrossRefGoogle ScholarPubMed
6.Bustin, SA, Benes, V, Garson, JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009;55:611–622.Google Scholar
7.National Molecular Subtyping Network for Foodborne Disease Surveillance. Oxacillin-Resistant Staphylococcus aureus on PulseNet (OPN): Laboratory Protocol for Molecular Typing of S.aureus by Pulsed-Field Gel Electrophoresis (PFGE). http://www.cdc.gov/ncidod/dhqp/pdf/ar/ar_mras_PFGE_s_aureus.pdf.Google Scholar
8.Clinical and Laboratory Standards Institute (CLSI). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 7th ed. Wayne, PA: CLSI, 2006. Approved standard M11-A7.Google Scholar
9.Benson, TE, Prince, DB, Mutchler, VT, et al. X-ray crystal structure of Staphylococcus aureus FemA. Structure 2002; 10: 1107–1115.Google Scholar
10.Liu, Q, Liu, M, Wu, Q, Li, C, Zhou, T, Ni, Y. Sensitivities to biocides and distribution of biocide resistance genes in quaternary ammonium compound tolerant Staphylococcus aureus isolated in a teaching hospital. Scand J Infect Dis 2009;41:403–409.Google Scholar