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Ribotyping and Random Amplification of Polymorphic DNA for Nosocomial Enterobacter cloacae Isolates in a Pediatric Intensive-Care Unit

Published online by Cambridge University Press:  02 January 2015

Shih-Ting Hou ,
Chih-Chien Wang  and
Mong-Ling Chu
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Abstract

Between 1987 and 1989, two sequential outbreaks of nosocomial infection caused by Enterobacter cloacae occurred in the pediatric intensive-care unit of a tertiary-care teaching hospital. Seventeen strains retrieved from the outbreaks and two control strains identified in other wards were typed by ribotyping and random amplification of polymorphic DNA (RAPD). The results indicated that the genomic pattern of strains identified between the first and second outbreaks was different. We conclude that both ribotyping and RAPD are highly discriminatory and reproducible methods for typing E cloacae. RAPD seems to be more efficacious and cost-effective.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 18 , Issue 11 , November 1997 , pp. 769 - 771
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1997

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