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Interinstitutional and Intrainstitutional Transmission of a Strain of Acinetobacter baumannii Detected by Molecular Analysis Comparison of Pulsed-Field Gel Electrophoresis and Repetitive Sequence–Based Polymerase Chain Reaction

Published online by Cambridge University Press:  21 June 2016

S. Saeed
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
M. G. Fakih
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
K. Riederer
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
A. R. Shah
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
R. Khatib*
Affiliation:
St. John Hospital & Medical Center, Detroit, Michigan
*
Medical Education, St. John Hospital & Medical Center, 22101 Moross Road, Detroit, MI 48236, ([email protected])

Abstract

Pulsed-field gel electrophoresis and repetitive sequence-based polymerase chain reaction provided comparable strain discrimination with minor discordance in typing Acinetobacter baumannii clinical isolates from patients at our hospital and affiliated institutions. Typing revealed a cluster strain with intrainstitutional and interinstitutional spread during the study period. A long-term acute care facility may have been the reservoir.

Type
Concise Communications
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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References

1.Bergogne-Berezin, E, Towner, KJ. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 1996; 9:148165.Google Scholar
2.Corbella, X, Montero, A, Pujol, M, et al. Emergence and rapid spread of carbapenem resistance during a large and sustained hospital outbreak of multiresistant Acinetobacter baumannii. J Clin Microbiol 2000; 38:40864095.CrossRefGoogle ScholarPubMed
3.Spence, RP, van der Reijden, TJ, Dijkshoorn, L, Towner, KJ. Comparison of Acinetobacter baumannii isolates from United Kingdom hospitals with predominant Northern European genotypes by amplified-fragment length polymorphism analysis. J Clin Microbiol 2004; 42:832834.Google Scholar
4.Wisplinghoff, H, Edmond, MB, Pfaller, MA, Jones, RN, Wenzel, RP, Seifert, H. Nosocomial bloodstream infections caused by Acinetobacter species in United States hospitals: clinical features, molecular epidemiology, and antimicrobial susceptibility. Clin Infect Dis 2000; 31:690697.CrossRefGoogle ScholarPubMed
5.Biendo, M, Laurans, G, Lefebvre, JF, Daoudi, F, Eb, F. Epidemiological study of an Acinetobacter baumannii outbreak by using a combination of antibiotyping and ribotyping. J Clin Microbiol 1999; 37:21702175.CrossRefGoogle ScholarPubMed
6.Quale, J, Bratu, S, Landman, D, Heddurshetti, R. Molecular epidemiology and mechanisms of carbapenems resistance in Acinetobacter baumannii endemic in New York City. Clin Infect Dis 2003; 37:214220.Google Scholar
7.Herruzo, R, de la Cruz, J, Fernandez-Acenero, MJ, Garcia-Caballero, J. Two consecutive outbreaks of Acinetobacter baumannii 1-A in a burn intensive care unit for adults. Burns 2004; 30:419423.Google Scholar
8.Poirel, L, Menuteau, O, Agoli, N, Cattoen, C, Nordmann, P. Outbreak of extended-spectrum beta-lactamase VEB-1-producing isolates of Acinetobacter baumannii in a French hospital. J Clin Microbiol 2003; 41:35423547.Google Scholar
9.Villegas, MV, Hartstein, AI. Acinetobacter outbreaks, 1977-2000. Infect Control Hosp Epidemiol 2003; 24:284295.Google Scholar
10.Manikal, VM, Landman, D, Saurina, G, Oydna, E, Lai, H, Quale, J. Endemic carbapenems-resistant Acinetobacter species in Brooklyn, New York: city-wide prevalence, interinstitutional spread, and relation to antibiotic usage. Clin Infect Dis 2000; 31:101106.Google Scholar