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Inactivation of Human Immunodeficiency Virus by a Medical Waste Disposal Process Using Chlorine Dioxide

Published online by Cambridge University Press:  21 June 2016

R. Wesley Farr  and
Cheryl Walton
R. Wesley Farr*
Affiliation:
Section of Infectious Diseases, West Virginia University, Morgantown, West Virginia
Cheryl Walton
Affiliation:
Section of Infectious Diseases, West Virginia University, Morgantown, West Virginia
*
Section of Infectious Diseases, Department of Medicine, PO Box 9163, West Virginia University, Morgantown, WV 26506-9163
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Abstract

Objective:

To study the ability of a medical waste disposal process using chlorine dioxide to inactivate human immunodeficiency virus type 1 (HIV 1).

Design:

Stock HIV-1 (HTLV-IIIB strain) was treated with chlorine dioxide under the following settings: cell culture medium alone, culture medium with 25% blood, culture medium with medical supplies treated by the Condor machine (Winfield Environmental Corp., Escondido, CA). MT-2 cells in 96-well tissue culture plates were inoculated with serial tenfold dilutions of treated and untreated HIV-1. Cytopathic effect was read on day five, and the TCID50 (50% tissue culture infectious dose) was calculated.

Results:

Treatment of HIV-1 with chlorine dioxide in culture medium alone resulted in a 5.25 log10 reduction in TCID50. Treatment of HIV-1 with chlorine dioxide in the presence of 25% blood caused a 6.25 log10 reduction in HIV-1 infectivity Treatment of HIV-1 with chlorine dioxide in the presence of medical supplies treated in the Condor machine resulted in a 4.75 log10 reduction in HIV infectivity.

Conclusions:

Chlorine dioxide inactivated HIV-1 in vitro. Chlorine dioxide inactivated HIV-1 in the presence of blood and in the presence of medical supplies under conditions that simulated the conditions existing in the Condor machine.

Type
Brief Report
Information
Infection Control & Hospital Epidemiology , Volume 14 , Issue 9 , September 1993 , pp. 527 - 529
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1993

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