Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-23T14:12:52.688Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection and Prevalence of Adenoviral Conjunctivitis among Hospital Employees Using Real-Time Polymerase Chain Reaction as an Infection Prevention Tool

Published online by Cambridge University Press:  10 May 2016

Irene C. Kuo ,
Colleen Espinosa ,
Michael Forman ,
Miriana Pehar ,
Lisa L. Maragakis  and
Alexandra Valsamakis
Irene C. Kuo*
Affiliation:
Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland
Colleen Espinosa
Affiliation:
Division of Occupational and Environmental Medicine, Johns Hopkins Hospital, Baltimore, Maryland
Michael Forman
Affiliation:
Division of Medical Microbiology, Department of Pathology, Johns Hopkins Hospital, Baltimore, Maryland
Miriana Pehar
Affiliation:
Department of Hospital Epidemiology and Infection Control, Johns Hopkins Hospital, Baltimore, Maryland
Lisa L. Maragakis
Affiliation:
Department of Hospital Epidemiology and Infection Control, Johns Hopkins Hospital, Baltimore, Maryland Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland
Alexandra Valsamakis
Affiliation:
Division of Medical Microbiology, Department of Pathology, Johns Hopkins Hospital, Baltimore, Maryland
*
Wilmer Eye Institute, 4924 Campbell Boulevard, #100, Baltimore, MD 21236 ([email protected]).
Get access Rights & Permissions [Opens in a new window]

Abstract

Hospital employees with suspected adenoviral conjunctivitis underwent evaluation and testing with real-time polymerase chain reaction. Viral conjunctivitis was suspected in 307 (59%) of 518 employees with eye complaints; adenovirus was detected in 4% (22 of 518). Four employees had genotypes consistent with epidemic keratoconjunctivitis. This algorithm minimizes productivity loss compared with clinical diagnosis.

Infect Control Hosp Epidemiol 2014;35(6):728–731

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 35 , Issue 6 , 01 June 2014 , pp. 728 - 731
Copyright
© 2014 by The Society for Healthcare Epidemiology of America. All rights reserved.

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Montessori, V, Scharf, S, Holland, S, Werker, DH, Roberts, FJ, Bryce, E. Epidemic keratoconjunctivitis outbreak at a tertiary referral eye care clinic. Am J Infect Control 1998;26(4):399–405.Google Scholar
2. Cheung, D, Bremner, J, Chan, JTK. Epidemic keratoconjunctivitis—do outbreaks have to be epidemic? Eye 2003;17:356–363.Google Scholar
3. Klapper, PE, Cleator, GM. Adenovirus cross-infection: a continuing problem. J Hosp Infect 1995;30(suppl):262–267.Google Scholar
4. Hamada, N, Gotoh, K, Hara, K, et al. Nosocomial outbreak of epidemic keratoconjunctivitis accompanying environmental contamination with adenoviruses. J Hosp Infect 2008;68:262–268.Google Scholar
5. Piednoir, E, Bureau-Chalot, F, Merle, C, Gotzmanis, A, Wulbout, J, Bajolet, O. Direct costs associated with a nosocomial outbreak of adenoviral conjunctivitis infection in a long-term care instititution. Am J Infect Control 2002;30:407–410.Google Scholar
6. Dart, JKG, El-Amir, AN, Maddison, T, et al. Identification and control of nosocomial adenovirus keratoconjunctivitis in an ophthalmic department. Br J Ophthalmol 2009;93:18–20.Google Scholar
7. Warren, D, Nelson, KE, Farrar, JA, et al. A large outbreak of epidemic keratoconjunctivitis. J Infec Dis 1989;160(6):938–943.Google Scholar
8. Gottsch, JD, Froggatt, JW, Smith, DM, et al. Prevention and control of epidemic keratoconjunctivitis in a teaching eye institute. Ophthalmic Epidemiol 1999;6:29–39.Google Scholar
9. Aoki, K, Kato, M, Ohtsuka, H, Ishii, K, Nakazono, N, Sawada, H. Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus type 4 and 19 infections. Br J Ophthalmol 1982;66:776–780.Google Scholar
10. Aoki, K, Kawana, R, Matsumoto, I, Wadell, G, de Jong, JC. Viral conjunctivitis with special reference to adenovirus type 37 and enterovirus 70 infection. Jpn J Ophthalmol 1986;30:158–164.Google Scholar
11. Aoki, K, Tagawa, Y. A twenty-one year surveillance of adenoviral conjunctivitis in Sapporo, Japan. Int Ophthalmol Clin 2002:42:49–54.Google Scholar
12. de Jong, JC, Wigand, R, Wadell, G, et al. Adenovirus 37: identification and characterization of a medically important new adenovirus type of subgroup D. J Med Virol 1981;7:105–118.Google Scholar
13. Desmyter, J, De Jong, JC, Slaterus, KW, Verlaeckt, H. Keratoconjunctivitis caused by adenovirus type 19. Br Med J 1974; 4:406.Google Scholar
14. Jawetz, E, Kimura, S, Nicholas, AN, Thygeson, P, Hanna, P. New type of APC virus from epidemic keratoconjunctivitis. Science 1955;122:1190–1191.Google Scholar
15. Wold, WSM, Horwitz, MS. Adenoviridae. In: Knipe, DM, Howley, PM, Griffin, DE, Lamb, RA, Martin, MA, Roizman, B, Straus, SE, eds. Fields virology. 5th ed. Vol. II. Philadelphia: Lippincott, Williams & Wilkins, 2007:2395–2436.Google Scholar
16. Heim, A, Ebnet, C, Harste, G, Pring-Akerblom, P. Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J Med Virol 2003;70:228–239.Google Scholar
17. Lu, X, Erdman, DD. Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Arch Virol 2006;151:1587–1602.Google Scholar