Preliminary multidisciplinary investigations were carried out in a 105 m thick late Pleistocene terrestrial sequence in the Lamayuru basin, Ladakh Himalaya. Palaeomagnetic studies reveal a reversal of polarity at about 35 ka BP. This is the only report of this reversal event in the late Pleistocene terrestrial sequences of the Indian subcontinent. The valley fill sequence is the product of an interplay of lacustrine with fluvio-deltaic to colluvial processes. Four fossiliferous horizons have yielded ostracods and gastropods indicating shallow freshwater conditions. The Lamayuru lake was created by tectonically induced instability about 35–40 ka BP, an event that may be represented widely throughout the Himalaya.