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A vector for studying plasmid stability functions in Streptomyces

Published online by Cambridge University Press:  14 April 2009

Kevin Kendall  and
John Cullum
Kevin Kendall
Affiliation:
Department of Biochemistry and Applied Molecular BiologyUniversity of Manchester Institute of Science and TechnologyP. O. Box 88 Manchester M60 1QD, U.K.
John Cullum*
Affiliation:
Department of Biochemistry and Applied Molecular BiologyUniversity of Manchester Institute of Science and TechnologyP. O. Box 88 Manchester M60 1QD, U.K.
*
† Correponding author

Summary

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We constructed a cloning vector (pMT603) based on the low copy number plasmid SCP2*. pMT6O3 is unstable because it lacks the SCP2* stability region and carries the selectable marker thiostrepton-resistance and a tyrosinase gene which results in melanin production. This allows easy testing of plasmid stability and we demonstrated its usefulness by cloning a plasmid stability function.

Type
Short Paper
Information
Genetics Research , Volume 51 , Issue 1 , February 1988 , pp. 71 - 74
Copyright
Copyright © Cambridge University Press 1988

References

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