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The use of retrotransposons as markers for mapping genes responsible for fitness differences between related Drosophila melanogaster strains

Published online by Cambridge University Press:  14 April 2009

Sergey V. Nuzhdin ,
Peter D. Keightley  and
Elena G. Pasyukova
Sergey V. Nuzhdin
Affiliation:
Institute of Molecular Genetics, Kurchatov Sq. 46, 123182, Moscow, Russia Department of Genetics, Box 7614, North Carolina State University, Raleigh, NC 27695, USA
Peter D. Keightley
Affiliation:
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland
Elena G. Pasyukova
Affiliation:
Institute of MolecularGenetics, Kurchatov Sq. 46, 123182, Moscow, Russia
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Hitch-hiking of dispersed mobile elements serving as molecular markers was used as a new tool for mapping quantitative trait loci in Drosophila melanogaster. Two Drosophila strains with high fitness (HA) were backcrossed repeatedly to a closely related strain with low fitness (LA) to initiate experimental populations with expected HA gene frequencies of 1/32. The frequencies of 19 insertion sites of the retrotransposons mdg1 and copia were analyzed after 11 to 17 generations. Frequencies of sites from the HA line increased substantially in the pericentromeric region, indicating that one or more loci responsible for the fitness difference between the strains were located there. A maximum likelihood (ML) procedure was applied to estimate selection coefficients associated with the markers, and this indicated a broad, strongly selected region of the chromosome. At least one additional locus was localized in the middle of the 2L arm. Possible applications of this method are discussed.

Type
Research Article
Information
Genetics Research , Volume 62 , Issue 2 , October 1993 , pp. 125 - 131
Copyright
Copyright © Cambridge University Press 1993

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