Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-19T06:33:22.778Z Has data issue: false hasContentIssue false
  • English
  • Français

Unequal crossing-over within the B duplication of Drosophila melanogaster: a molecular analysis

Published online by Cambridge University Press:  14 April 2009

Stuart I. Tsubota
Stuart I. Tsubota
Affiliation:
Department of Biology, University of Michigan, Ann Arbor, Michigan 49109, USA
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The B mutation is associated with a tandem duplication of 16A1–16A7. It is unstable, mutating to wild type and to a more extreme form at a frequency of one in 1000 to 3000. The reversion to wild type is associated with the loss of one copy of the duplication, whereas the mutation to extreme B is associated with a triplication of the region. The instability of B has been attributed to unequal crossing-over between the two copies of the duplication. Recent molecular data show that there is a transposable element, B104, between the two copies of the duplication and support the hypothesis that this element generated the duplication via a recombination event. These data suggest that unequal crossing-over within the duplication may not be the cause of the instability of B. Instead, the instability may be caused by a recombination event involving the B104 element. This issue was addressed using probes for the DNA on either side of the B104 element at the B breakpoint. All of the data indicate that the B104 element is not involved in the instability of B and support the original unequal crossing-over model.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 2 , April 1991 , pp. 105 - 111
Copyright
Copyright © Cambridge University Press 1991

References

Bridges, C. B. (1936). The Bar ‘gene’ a duplication. Science 83, 210–211.CrossRefGoogle ScholarPubMed
Davis, P. S., Shen, M. W. & Judd, B. H. (1987). Asymmetrical pairings of transposons in and proximal to the white locus of Drosophila account for four classes of regularly occurring exchange products. Proceedings of the National Academy of Science, USA 84, 174–178.CrossRefGoogle Scholar
Gabay, S. J. & Laughnan, J. R. (1970). Genetic analysis of the aberrant behavior of an X-chromosome duplication in the germ line of Drosophila melanogaster males. Genetics 65, 249–265.CrossRefGoogle ScholarPubMed
Green, M. M. (1968). Some genetic properties of intrachromosomal recombination. Molecular and General Genetics 103, 209–217.CrossRefGoogle ScholarPubMed
Lindsley, D. L. & Grell, E. H. (1968). Genetic variations of Drosophila melanogaster. Carnegie Institution of Washington Publication No. 627.Google Scholar
Muller, H. J., Prokofyeva-Belgovskaya, A. A. & Kossikov, K. V. (1936). Unequal crossing-over in the Bar mutant as a result of duplication of a minute chromosome section. Doklady Akademii Nauk SSSR 1, 87–88.Google Scholar
Peterson, H. M. & Laughnan, J. R. (1963). Intrachromosomal exchange at the Bar locus in Drosophila. Proceedings of the National Academy of Science, USA 50, 126–133.CrossRefGoogle ScholarPubMed
Peterson, H. M. & Laughnan, J. R. (1964). Premeiotic exchange within a duplication-X chromosome in Drosophila melanogaster males. Genetics 50, 275–276.Google Scholar
Scherer, G., Tschudi, C., Perera, J., Delius, H. & Pirrotta, V. (1982). B104, a new dispersed repeated gene family in Drosophila melanogaster and its analogies with retroviruses. Journal of Molecular Biology 157, 435–451.CrossRefGoogle ScholarPubMed
Sturtevant, A. H. (1925). The effects of unequal crossing over at the Bar locus in Drosophila. Genetics 10, 117–147.Google Scholar
Sutton, E. (1943). Bar eye in Drosophila melanogaster: a cytological analysis of some mutations and reverse mutations. Genetics 28, 97–107.CrossRefGoogle ScholarPubMed
Tice, S. C. (1914). A new sex-linked character in Drosophila. Biological Bulletin 26, 221–230.CrossRefGoogle Scholar
Tsubota, S. I., Rosenberg, D., Szostak, H., Rubin, D. & Schedl, P. (1989). The cloning of the Bar region and the B breakpoint in Drosophila melanogaster: evidence for a transposon-induced rearrangement. Genetics 122, 881–890.CrossRefGoogle Scholar
Tsubota, S. I. & Schedl, P. (1986). Hybrid-dysgenesis-induced revenants of insertions at the 5′ end of the rudimentary gene in Drosophila melanogaster: transposon-induced control mutations. Genetics 114, 165–182.CrossRefGoogle Scholar
Zeleny, C. (1919). A change in the bar gene of Drosophila involving further decrease in facet number and increase in dominance. Journal of General Physiology 2, 69–71.CrossRefGoogle ScholarPubMed
Zeleny, C. (1921). The direction and frequency of mutation in the bar-eye series of multiple allelomorphs of Drosophila. Journal of Experimental Zoology 34, 203–233.Google Scholar