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Transmission of a genetic trait through total conjugation in a hypotrich ciliate Paraurostyla weissei. Genetic basis of the multi-left-marginal mutant

Published online by Cambridge University Press:  14 April 2009

Maria Jerka-Dziadosz  and
Bozena Dubielecka
Maria Jerka-Dziadosz
Affiliation:
Department of Cell Biology, M. Nencki Institute of Experimental Biology, Polish Academy of Science, Warsaw, Poland
Bozena Dubielecka
Affiliation:
Department of Cell Biology, M. Nencki Institute of Experimental Biology, Polish Academy of Science, Warsaw, Poland
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Summary

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The genetic basis of slow growth rate and aberrations in the ciliary pattern was studied in the multi-left-marginal variant of Paraurostyla weissei. The 3:1 segregation in F2 sibling crosses and 1:1 segregation in test crosses indicate that the aberrant phenotype is controlled by a recessive allele at a single gene locus termed mlm. The phenotypic change from wild type to mlm takes place about 5–8 cell cycles after conjugation. The study established that total conjugation in P. weissei is a true sexual process in which meiosis, fertilization and Mendelian segregation occur.

Type
Research Article
Information
Genetics Research , Volume 46 , Issue 3 , December 1985 , pp. 263 - 271
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Aufderheide, K. J., Frankel, J. & Williams, N. E. (1980). Formation and positioning of surface-related structures in Protozoa. Microbiological Reviews 44, 252–302.CrossRefGoogle ScholarPubMed
Berger, J. (1976). Gene expression and phenotypic change in Paramecium tetraurelia exconjugants. Genetical Research 27, 123–134.CrossRefGoogle ScholarPubMed
Bleyman, L. K. & Simon, E. M. (1967). Genetic control of maturity in Tetrahymena pyriformis. Genetical Research 10, 319–321.CrossRefGoogle ScholarPubMed
Dubielecka, B. & Jerka-Dziadosz, M. (1984). Genetic basis of aberration in the cortical pattern in mlm mutant of the hypotrich ciliate Paraurostyla weissei. Postepy Biologii Komórki 11, 607–610.Google Scholar
Frankel, J. (1973 a). Dimensions of cortical patterns in Euplotes: the role of pre-existing structures, the clonal life cycle and the genotype. Journal of Experimental Zoology 183, 71–94.CrossRefGoogle Scholar
Frankel, J. (1973 b). A genically determined abnormality in the number and arrangement of basal bodies in a ciliate. Developmental Biology 30, 336–365.CrossRefGoogle Scholar
Frankel, J. & Jenkins, L. (1979). A mutant of Tetrahymena thermophila with a partial mirror-image duplication of cell surface pattern. II. Nature of genic control. Journal of Embryology and Experimental Morphology 49, 203–227.Google ScholarPubMed
Frankel, J. & Nelsen, E. M. (1981). Discontinuities and overlaps in patterning within single cells. Philosophical Transaction of Royal Society of London, B 295, 525–538.Google ScholarPubMed
Frankel, J. & Nelsen, M. E. (1985). Intracellular pattern reversal in Tetrahymena thermophila. Transient expression of a janus phenocopy in balanced doublets. Developmental Biology. Submitted.Google Scholar
Heckmann, K. (1963). Paarungssystem und genabhängige Paarungstyp-differenzierung bei dem hypotrichen Ciliaten Euplotes vannus O. F. Müller. Archiv für Protistenkunde 106, 393–421.Google Scholar
Heckmann, K. (1965). Total Konjugation bei Urostyla hologama sp.n. Archiv für Protistenkunde 108, 55–62.Google Scholar
Heckmann, K. & Frankel, J. (1968). Genic control of cortical pattern in Euplotes. Journal of Experimental Zoology 168, 11–38.CrossRefGoogle ScholarPubMed
Heumann, J. (1975). Conjugation of the hypotrich ciliate Paraurostyla weissei (Stein). A scanning electron microscope study. Journal of Protozoology 22, 392–397.CrossRefGoogle Scholar
Jerka-Dziadosz, M. (1976). The proportional regulation of cortical structures in a hypotrich ciliate Parauostyla weissei. Journal of Experimental Zoology 195, 1–14.CrossRefGoogle Scholar
Jerka-Dziadosz, M. (1982) Ultrstructural study on development of the hypotrich ciliate Paraurostyla weissei. IV. Morphogenesis of dorsal bristles and caudal cirri. Protistologica 18, 237–251.Google Scholar
Jerka-Dziadosz, M. (1984). Organization of microtubules in subcortical cytoskeleton during life-cycle of the protozoan ciliate Paraurostyla weissei. Cortical microtubules in total conjugation. Abstract no. 4221, 3rd International Congress on Cell Biology, 08 26–31, 1984, Tokyo, Japan.Google Scholar
Jerka-Dziadosz, M. (1985). Mirror-image configuration of the cortical pattern causes modifications in propagation of microtubular structures in hypotrich ciliate Paraurostyla weissei. Roux's Archives of Developmental Biology 194, 311–324.CrossRefGoogle Scholar
Jerka-Dziadosz, M. & Banaczyk, I. A. (1983). Cell shape, growth rate and cortical pattern aberrations in an abnormal strain of the hypotrich ciliate Paraurostyla weissei. Acta Proto-zoologica 22, 139–156.Google Scholar
Jerka-Dziadosz, M. & Frankel, J. (1969). An analysis of the formation of ciliary primordia in the hypotrich ciliate Urostyla weissei. Journal of Protozoology 16, 612–637.CrossRefGoogle ScholarPubMed
Jerka-Dziadosz, M. & Frankel, J. (1979). A mutant of Tetrahymena with a partial mirror-image duplication of cell surface pattern. Analysis of the phenotype. Journal of Embryology and Experimental Morphology 49, 167–202.Google ScholarPubMed
Jerka-Dziadosz, M. & Janus, I. (1975). Discontinuity of cortical pattern during total conjugation of a hypotrich ciliate Paraurostyla weissei. Acta Protozoologica 13, 309–332.Google Scholar
Kaczanowski, A. (1975). A single-gene dependent abnormality of adoral membranelles in Tetrahymena pyriformis, species 1. Genetics 81, 631–639.CrossRefGoogle ScholarPubMed
Ng, S. F. (1976). Extra-cytoproct mutant in Paramecium tetraurelia, a genetical study. Genetical Research 27, 143–159.CrossRefGoogle ScholarPubMed
Nanney, D. (1980). Experimental Ciliatology. New York: John Wiley.Google Scholar
Sapra, G. R. & Ammermann, D. (1973). RNA synthesis and acquisition of actinomycin D insensitivity during conjugation in Stylonychia mytilus. Experimental Cell Research 78, 168–174.CrossRefGoogle ScholarPubMed
Sapra, G. R. & Ammermann, D. (1974). An analysis of the development program in relation to RNA metabolism in the ciliate Stylonychia mytilus. Developmental Biology 36, 105–112.CrossRefGoogle ScholarPubMed