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Transformation in Escherichia coli: studies on the nature of donor DNA after uptake and integration

Published online by Cambridge University Press:  14 April 2009

W. P. M. Hoekstra ,
H. E. N. Bergmans  and
E. M. Zuidweg
W. P. M. Hoekstra
Affiliation:
Department of Molecular Cell Biology, State University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
H. E. N. Bergmans
Affiliation:
Department of Molecular Cell Biology, State University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
E. M. Zuidweg
Affiliation:
Department of Molecular Cell Biology, State University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands

Summary

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Chromosomal E. coli DNA appears to be sensitive towards in vivo DNA restriction when transformed to a restrictive E. coli recipient. It is therefore concluded that transforming chromosomal donor DNA is present in a double-stranded form immediately after uptake.

Genetic analysis of E. coli transformants, obtained with UV-irradiated donor DNA under conditions that exclude photorepair, show, especially in a uvrB recipient, loss of donor DNA information compared with the situation where DNA was not subjected to UV-irradiation. Similar conclusions were arrived at after genetic analysis of transductants obtained with UV-irradiated particles of the generalized transducing phage P1. The processing in E. coli of DNA after P1 transduction is thus similar to that of transforming DNA. The observations are discussed and a possible explanation based on single-stranded DNA integration is presented in detail.

Type
Research Article
Information
Genetics Research , Volume 35 , Issue 3 , June 1980 , pp. 279 - 289
Copyright
Copyright © Cambridge University Press 1980

References

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