Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-23T00:40:36.787Z Has data issue: false hasContentIssue false
  • English
  • Français

Transfer-positive J-independent revertants of the F factor in Escherichia coli K 12

Published online by Cambridge University Press:  14 April 2009

Mark Achtman
Mark Achtman
Affiliation:
M.R.C. Molecular Genetics Research Unit, Department of Molecular Biology, University of Edinburgh, Edinburgh, Scotland
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Cells carrying traJ− mutants of F are transfer-deficient and are good recipients in conjugation (Achtman, Willets & Clark, 1972). In addition, the traJ gene product is involved in pilus- and in plasmid-specificity (Willetts, 1971). J-independent mutants were isolated as revertants of a traJ− mutant; they still carry the traJ− mutation but also carry at least one other mutation which results in transfer in the absence of the traJ gene product. J-independent transfer of these mutants is not inhibited by the R 100 repressor. Various models are presented which can account for the properties of traJ− mutants and of these J-independent revertants.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 1 , February 1973 , pp. 67 - 77
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Achtman, M., Willetts, N. S. & Clark, A. J. (1971). Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants, Journal of Bacteriology 106, 529–538.CrossRefGoogle Scholar
Achtman, M., Willetts, N. S. & Clark, A. J. (1972). A conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli. Journal of Bacteriology 110, 831–842.CrossRefGoogle ScholarPubMed
Adams, M. H. (1959). Bacteriophages. New York: Interscience Publishers, Inc.CrossRefGoogle Scholar
Barbour, S. D., Nagaishi, H., Templin, A. & Clark, A. J. (1970). Biochemical and genetic studies of recombination proficiency in E. coli. II. Rec+ revertants caused by indirect suppression of Rec− mutations. Proceedings of the National Academy of Sciences of the United States of America. 67, 128–135.CrossRefGoogle Scholar
Butler, B. & Echols, H. (1970). Regulation of bacteriophage A development by gene N: properties of a mutation that bypasses N control of late protein synthesis. Virology 40, 212–222.CrossRefGoogle Scholar
Court, D. & Sato, K. (1969). Studies of novel transducing variants of bacteriophage A: dispensibility of genes N and Q. Virology 39, 348–352.CrossRefGoogle Scholar
Demerec, M., Adelberg, B. A., Clark, A. J. & Hartman, P. E. (1966). A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76.CrossRefGoogle ScholarPubMed
Egawa, R. & Hirota, Y. (1962). Inhibition of fertility by multiple drug resistance factors in E. coli. Japan. Journal of Genetics 37, 66–69.Google Scholar
Englesberg, E., Sheppard, D., Squires, C. & Meronk, F. Jr (1969). An analysis of ‘revertants’ of a deletion mutant in the C gene of the L-arabinose gene complex in Escherichia coli B/r: isolation of initiator constitutive mutants. Journal of Molecular Biology 43, 281–298.CrossRefGoogle Scholar
Finnegan, D. J. & Willetts, N. S. (1971). Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Molecular General Genetics 111, 256–264.CrossRefGoogle ScholarPubMed
Hofnung, M. & Schwartz, M. (1971). Mutation allowing growth on maltose of E. coli K12 strains with a deleted mal T gene. Molecular General Genetics 112, 117–132.CrossRefGoogle Scholar
Hopkins, N. (1970). Bypassing a positive regulator: isolation of a λ mutant that does not require N product to grow. Virology 40, 223–229.CrossRefGoogle Scholar
Ippen-Ihler, K., Achtman, M. & Willetts, N. (1972). A deletion map of the E. coli sex factor F: the order of eleven transfer cistrons. Journal of Bacteriology 110, 857–863.CrossRefGoogle Scholar
Kemper, J. & Margolin, P. (1969). Suppression by gene substitution for the leuD gene of S. typhimurium. Genetics 63, 263–279.CrossRefGoogle Scholar
Luria, S. E. & Burrous, J. W. (1957). Hybridization between E. coli and Shigella. Journal of Bacteriology 74, 461–476.CrossRefGoogle Scholar
Luria, S. E. & Delbruck, M. (1943). Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28, 491–511.CrossRefGoogle ScholarPubMed
Nishimura, Y., Ishibashi, M., Meynell, E. & Hirota, Y. (1967). Specific piliation directed by a fertility factor and a resistance factor of E. coli. Journal of General Microbiology 49, 89–98.CrossRefGoogle Scholar
Ohtsubo, E. (1970). Transfer defective mutants of sex factors in E. coli. II. Deletion mutants of an F prime and deletion mapping of cistrons involved in genetic transfer. Genetics 64, 189–197.CrossRefGoogle Scholar
Ohtsubo, E., Nishimura, Y. & Hirota, Y. (1970). Transfer defective mutants of sex factors in E. coli. I. Defective mutants and complementation analysis. Genetics 64, 173–188.CrossRefGoogle Scholar
Taylor, L. (1970). Current linkage map of E. coli. Bacteriological Reviews 34, 155–175.CrossRefGoogle Scholar
Watanabe, T. & Fukasawa, T. (1962). Episome mediated transfer of drug resistance in Enterobacteriaceae. IV. Interactions between resistance transfer factors and F-factors in E. coli K12. Journal of Bacteriology 83, 727–735.CrossRefGoogle Scholar
Willetts, N. S. (1971). Plasmid specificity of two proteins required for conjugation in E. coli K12. Nature New Biology 230, 183–185.CrossRefGoogle ScholarPubMed
Willetts, N. S. & Achtman, M. (1972). A genetic analysis of transfer by the E. coli sex factor F, using P1 transductional complementation. Journal of Bacteriology 110, 843–851.CrossRefGoogle Scholar
Willetts, N. S. & Finnegan, D. J. (1970). Characteristics of E. coli K12 strains carrying both an F prime and an R factor. Genetical Research 16, 113–122.CrossRefGoogle Scholar