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Transcriptional organization of the Tra2 region controlling conjugational transfer of the narrow host range Pseudomonas aeruginosa plasmid R91–5

Published online by Cambridge University Press:  14 April 2009

R. J. Moore  and
V. Krishnapillai
R. J. Moore
Affiliation:
Department of Genetics, Monash University Clayton, Victoria 3168, Australia
V. Krishnapillai
Affiliation:
Department of Genetics, Monash University Clayton, Victoria 3168, Australia
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The transcriptional organization of the Tra2 region controlling conjugation by the narrow host range plasmid R91–5 of Pseudomonas aeruginosa has been determined. This region of 15·2 kilobase pairs of DNA encodes ten cistrons and specifies the synthesis and functionality of sex pili. The order of the cistrons was previously shown to be traW, (S, Z), U, (V, R), Q, T, Y, X (those within parentheses could not be ordered with respect to each other). The organization was determined by complementation tests for the restoration of plasmid transfer between transposon Tn7 or Tn501 induced insertion mutants and point mutants representative of the ten cistrons. Polarity mutations induced by Tn7 permitted the identification of three operons. Cistrons traW, Z, S, U, V, R and Q formed a large operon with transcription postulated to be in that order. The second consisted of tra T and tra Y also in that order and the third consisted of only one cistron, traX. Polarity was not observed with Tn501 insertions as they still permitted full expression of cistrons distal to the site of insertion with respect to the proposed promoter. Despite the unexpected behaviour of this transposon it nevertheless led to the identification of two previously unidentified cistrons, traO and traP being between traZ and S and traR and V, respectively.

Type
Research Article
Information
Genetics Research , Volume 45 , Issue 2 , April 1985 , pp. 119 - 125
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Beutin, L., Manning, P. A., Achtman, M. & Willetts, N. (1981). sfrA and sfrB products of Escherichia coli K-12 are transcriptional control factors. Journal of Bacteriology 145, 840–844.CrossRefGoogle ScholarPubMed
Bradley, D. E. (1980). Morphological and serological relationships of conjugative pili. Plasmid 4, 155–169.CrossRefGoogle ScholarPubMed
Bradley, D. E. (1983). Specification of the conjugative pili and surface mating systems of Pseudomonas plasmids. Journal of General Microbiology 129, 2545–2556.Google ScholarPubMed
Carrigan, J. M. & Krishnapillai, V. (1979). Identification of cistrons involved in conjugal transfer of narrow host range R plasmid R91–5 of Pseudomonas aeruginosa. Journal of Bacteriology 140, 809–816.CrossRefGoogle ScholarPubMed
Helmuth, R. & Achtman, M. (1975). Operon structure of DNA transfer cistrons on the F sex factor. Nature 257, 652–656.CrossRefGoogle ScholarPubMed
Kleckner, N. (1981). Transposable elements in prokaryotes. Annual Review of Genetics 15, 341–404.CrossRefGoogle ScholarPubMed
Krishnapillai, V. (1971). A novel transducing phage: its role in recognition of a possible new host controlled modification system in Pseudomonas aeruginosa. Molecular and General Genetics 114, 134–143.CrossRefGoogle Scholar
Moore, R. J. (1981). The physical and genetic organization of the Pseudomonas aeruginosa plasmid R91–5. Ph.D. Thesis. Monash University, Melbourne, Australia.Google Scholar
Moore, R. J. & Krishnapillai, V. (1982 a). Tn7 and Tn501 insertions into Pseudomonas aeruginosa plasmid R91–5: Mapping of two transfer regions. Journal of Bacteriology 149 276–283.CrossRefGoogle ScholarPubMed
Moore, R. J. & Krishnapillai, V. (1982 b). Physical and genetic analysis of deletion mutants of plasmid R91–5 and the cloning of transfer genes in Pseudomonas aeruginosa. Journal of Bacteriology 149, 284–293.CrossRefGoogle ScholarPubMed
Willetts, N. & Skurray, R. (1980). The conjugation system of F-like plasmids. Annual Review of Genetics 14, 41–76.CrossRefGoogle ScholarPubMed