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Synchrony of division in mass cultures of Paramecium tetraurelia by a selective density-labelling technique*

Published online by Cambridge University Press:  14 April 2009

Karl Aufderheide
Karl Aufderheide
Affiliation:
Department of Zoology, Jordan Hall, Indiana University, Bloomington IN 47401, U.S.A.
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Summary

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A technique has been developed for achieving synchrony of division by selection for immediate pre- and post-fission cells of Paramecium tetraurelia. Cell populations are fed granular tantalum, followed by density-gradient centrifugation. Dividing cells, which do not feed, are separated from feeding interphase cells. The selected cells are division-synchronous for at least two generations when resuspended in fresh culture fluid. This technique provides a rapid means of obtaining large numbers of synchronously dividing paramecia for cell-cycle studies and has general application for selective enrichment of non-feeding sub-populations of cells.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 171 - 177
Copyright
Copyright © Cambridge University Press 1976

References

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