Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-20T07:22:06.085Z Has data issue: false hasContentIssue false
  • English
  • Français

Suppressor-specificity of antisuppressors in yeast

Published online by Cambridge University Press:  14 April 2009

Shirley J. McCready  and
Brian Cox
Shirley J. McCready
Affiliation:
Botany School, Oxford
Brian Cox
Affiliation:
Botany School, Oxford
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Eighteen mutations of Saccharomyces cerevisiae, at eight loci, isolated as antisuppressors of SUPQ2, an ochre-suppressing allele of SUP11, were crossed with three other suppressors.

They were found to abolish the ability of SUP2 (inserting tyrosine), to suppress the ochre mutations ade2.1 and can1.100, but not its ability, to suppress his5.2 or lys1.1. When coupled with any antisuppressor, SUPQ5, inserting serine, was also unable to suppress ade2.1, but the suppression of other ochre mutations varied from one asu-SUPQ5 strain to another. No antisuppressor affected the ability of SUP11-am, an amber-suppressing allele of SUP11, to suppress trp1.1, an amber mutation.

Type
Research Article
Information
Genetics Research , Volume 28 , Issue 2 , October 1976 , pp. 129 - 138
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Bruenn, J. & Jacobsen, B. (1972). New species of tyrosine tRNA in nonsense suppressor strains of yeast. Biochim. Biophys. Acta 287, 68–76.Google Scholar
Capecchi, M. R., Hughes, S. H. & Wahl, G. M. (1975). Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro. Cell 6, 269–277.Google Scholar
Cox, B. S. (1965). Ψ: a cytoplasmic suppressor of super-suppressor in yeast. Heredity 20, 505–521.Google Scholar
Cox, B. S. (1971). A recessive lethal super-suppressor mutation in yeast and other Ψ phenomena. Heredity 26, 211–232.CrossRefGoogle ScholarPubMed
Fittler, F. & Hall, R. H. (1966). Selective modification of yeast seryl-t-RNA and its effect on the acceptance and binding functions. Biochem. Biophys. Res. Comm. 25, 441–446.Google Scholar
Gilmore, R. A., Stewart, J. W. & Sherman, F. (1971). Amino-acid replacements resulting from supersuppression of nonsense mutants of iso-1-cytochrome c from yeasts. J. molec. Biol. 61, 157–173.Google Scholar
Hartwell, L. H. & McLaughlin, C. S. (1968). Mutants of yeast with temperature-sensitive isoleucyl-tRNA synthetases. Proc. natn. Acad. Sci. U.S.A. 59, 422–428.Google Scholar
Hawthorne, D. C. & Mortimer, R. K. (1968). Genetic mapping of nonsense suppressors in yeast. Genetics 60, 735–742.CrossRefGoogle ScholarPubMed
Hirsh, D. (1971). Tryptophan transfer RNA as the UGA suppressor. J. molec. Biol. 58, 439–458.Google Scholar
Lagerkvist, U. & Waldenström, J. (1965). Some properties of purified valyl and lysyl ribonucleic acid synthetase from yeast. J. biol. Chem. 240, 2246–2265.CrossRefGoogle ScholarPubMed
Liebman, S. W., Stewart, J. W. & Sherman, F. (1975). Serine substitutions caused by an ochre-specific suppressor in yeast. J. molec. Biol. 94, 595–610.Google Scholar
McCready, S. J. & Cox, B. S. (1973). Antisuppressors in yeast. Molec. gen. Genetics 124, 305–320.Google Scholar
Makman, M. H. & Cantoni, G. L. (1965). Isolation of seryl- and phenylalanyl-ribonucleic acid synthetases from baker's yeast. Biochemistry 4, 1434–1442.CrossRefGoogle Scholar
Sherman, F., Liebman, S. W., Stewart, J. W. & Jackson, M. (1973). Tyrosine substitutions resulting from suppression of amber mutants of iso-1-cytochrome c in yeast. J. molec. Biol. 78, 157–168.Google Scholar
Yoshida, M., Takekhi, K. & Ukita, T. (1971). Structural studies on a yeast glutamic acid tRNA specific to GAA codon. Biochim. Biophys. Acta 228, 153–166.Google Scholar