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Suppressors of ‘blue’ mutations in yeast

Published online by Cambridge University Press:  14 April 2009

I. de G. Mitchell  and
E. A. Bevan
I. de G. Mitchell
Affiliation:
Department of Plant Biology and Microbiology, Queen Mary College, London University, London, E.1
E. A. Bevan
Affiliation:
Department of Plant Biology and Microbiology, Queen Mary College, London University, London, E.1
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Mutants of Saccharomyces cerevisiae whose colonies were blue when grown on nutrient agar medium containing methylene blue reverted to wild-type with white-colony phenotype at high frequency. This reversion was controlled by nuclear gene suppressors in some mutants, and by cytoplasmic suppressors in others. Each of the latter suppressed several independetly segregating blue mutants. These suppressors could be divided into two classes: suppression by petite mutations which behaved as recessives, and suppression by a cytoplasmic factor in respiration-sufficient cells which behaved as dominant over wild-type but might also be a mutation of rho. A relationship between blue mutation and temperature sensitivity was suggested.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 1 , February 1974 , pp. 37 - 45
Copyright
Copyright © Cambridge University Press 1974

References

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