Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-26T15:07:35.325Z Has data issue: false hasContentIssue false
  • English
  • Français

Superinfection inhibition by prophage B3 of some R plasmids in Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

V. Krishnapillai
V. Krishnapillai
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The prophage of the phage B3 of P. aeruginosa exhibits superinfection inhibition towards certain R plasmids such as R18-1 or R18-3 as shown by a severe depression (c. 10−6) in the conjugal transfer of these plasmids into B3 lysogens. The transfer of other R plasmids into such lysogens and the transfer of R18-1 or R18-3 into bacteria lysogenic for unrelated phages was unimpaired. R18-1 or R18-3 was non-transducible into B3 lysogens although transducible into non-lysogens. The interaction appeared to be reciprocally expressed since B3 lysogenization of bacteria R+ for R18-1 or R18-3 was also reduced. Consistent with the failure of co-existence of the prophage and the plasmids was the observation that when the transconjugants from the cross R18-1 donor ×B3 lysogenic recipients were tested, transfer-deficient plasmid derivatives were encountered (about 40%). Other transconjugants were cured of prophage, or carried mutant B3 prophage with lytic, lysogenization or superinfection inhibition functions impaired.

Type
Research Article
Information
Genetics Research , Volume 29 , Issue 1 , February 1977 , pp. 47 - 54
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience.CrossRefGoogle Scholar
Arber, W. (1960). Transduction of chromosomal genes and episomes in Escherichia coli. Virology 11, 273–288.CrossRefGoogle ScholarPubMed
Carey, K. E. (1974). Genetic and physiological studies of phages of Pseudomonas aeruginosa. Ph.D. thesis, Monash University, Victoria, Australia.Google Scholar
Chandler, P. M. & Krishnapillai, V. (1974 a). Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors readily transferable between Pseudomonas and the Enterobacteriaceae. Genetical Research 23, 239–250.CrossRefGoogle ScholarPubMed
Chandler, P. M. & Krishnapillai, V. (1974 b). Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors transferable only in Pseudomonas aeruginosa. Genetical Research 23, 251–257.CrossRefGoogle ScholarPubMed
Chandler, P. M. (1975). Pseudomonas aeruginosa R factors. Ph.D. thesis, Monash University, Victoria, Australia.Google Scholar
Datta, N., Hedges, R. W., Shaw, E. J., Sykes, R. B. & Richmond, M. H. (1971). Properties of an R factor from Pseudomonas aeruginosa. Journal of Bacteriology 108, 1244–1249.CrossRefGoogle Scholar
Datta, N. (1975). Microbiology – 1974 (ed. Schlessinger, D.), pp. 9–15. Washington, D.C.: American Society for Microbiology.Google Scholar
De Vries, J. K. & Maas, W. K. (1973). Description of an incompatibility mutant of Escherichia coli. Journal of Bacteriology 115, 213–220.CrossRefGoogle ScholarPubMed
Grinsted, J., Saunders, J. R., Ingram, L. C., Sykes, R. B. & Richmond, M. H. (1972). Properties of an R factor which originated in Pseudomonas aeruginosa 1822. Journal of Bacteriology 110, 529–537.CrossRefGoogle Scholar
Hedges, R. W., Jacob, A. E., Barth, P. T. & Grinter, N. J. (1975). Compatibility properties of P1 and ø AMP prophages. Molecular and General Genetics 141, 263–268.CrossRefGoogle Scholar
Holloway, B. W. (1969). Genetics of Pseudomonas. Bacteriological Reviews 33, 419–443.CrossRefGoogle ScholarPubMed
Holloway, B. W. & Richmond, M. H. (1973). R factors used for genetic studies in strains of Pseudomonas aeruginosa and their origin. Genetical Research 21, 103–105.CrossRefGoogle Scholar
Ingram, L., Sykes, R. B., Grinsted, J., Saunders, J. R. & Richmond, M. H. (1972). A transmissible resistance element from a strain of Pseudomonas aeruginosa containing no detectable extrachromosomal DNA. Journal of General Microbiology 72, 269–279.CrossRefGoogle ScholarPubMed
Isaac, J. H. & Holloway, B. W. (1968). Control of pyrimidine biosynthesis in Pseudomonas aeruginosa. Journal of Bacteriology 96, 1732–1741.CrossRefGoogle ScholarPubMed
Jacoby, G. A. (1974). Properties of an R plasmid in Pseudomonas aeruginosa producing amikacin (DB-K8), butirosin, kanamycin, tobramycin, and sisomycin resistance. Antimicrobial Agents and Chemotherapy 6, 807–810.CrossRefGoogle Scholar
Jacoby, G. A. (1976). Plasmids of Pseudomonas. In DNA Insertion Elements, Plasmids and Episomes to be published by the Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (submitted).Google Scholar
Krishnapillai, V. (1971). A novel transducing phage: Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa. Molecular and General Genetics 114, 134–143.CrossRefGoogle Scholar
Krishnapillai, V. (1974). The use of bacteriophages for differentiating plasmids of Pseudomonas aeruginosa. Genetical Research 23, 327–334.CrossRefGoogle ScholarPubMed
Lowbury, E. J. L., Kidson, A., Lilly, H. A., Ayliffe, G. A. J. & Jones, R. J. (1969). Sensitivity of Pseudomonas aeruginosa to antibiotics and emergence of strains highly resistant to carbenicillin. Lancet ii, 448–452.CrossRefGoogle Scholar
Novice, R. P., Clowes, R. C., Cohen, S. N., Curtiss, R. III, Datta, N. & Falkow, S. (1976). Uniform nomenclature for bacterial plasmids: a proposal. Bacteriological Reviews 40, 168–189.CrossRefGoogle Scholar
Richmond, M. H. & Sykes, R. B. (1972). The chromosomal integration of a β-lactamase gene derived from the P-type R factor RP1 in Escherichia coli. Genetical Research 20, 231–237.CrossRefGoogle ScholarPubMed
Schwesinger, M. D. & Novick, R. P. (1975). Prophage-dependent plasmid integration in Staphylococcus aureus. Journal of Bacteriology 123, 724–738.CrossRefGoogle ScholarPubMed
Stanisich, V. A. (1974). The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. Journal of General Microbiology 84, 332–334.CrossRefGoogle ScholarPubMed
Wyman, L. & Novick, R. P. (1974). Studies on plasmid replication. IV. Complementation of replication-defective mutants by an incompatibility-deficient plasmid. Molecular and General Genetics 135, 149–161.CrossRefGoogle ScholarPubMed