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Studies of esterase 6 in Drosophila melanogaster: XIV. Variation of esterase 6 levels controlled by unlinked genes in natural populations

Published online by Cambridge University Press:  14 April 2009

Craig S. Tepper ,
Anne L. Terry ,
James E. Holmes  and
Rollin C. Richmond
Craig S. Tepper
Affiliation:
Department of Biology, Indiana University, Bloomington, IN 47405, U.S.A.
Anne L. Terry
Affiliation:
Department of Biology, Indiana University, Bloomington, IN 47405, U.S.A.
James E. Holmes
Affiliation:
Department of Biology, Indiana University, Bloomington, IN 47405, U.S.A.
Rollin C. Richmond
Affiliation:
Department of Biology, Indiana University, Bloomington, IN 47405, U.S.A.
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The esterase 6 (Est-6) locus in Drosophila melanogaster is located on the third chromosome and is the structural gene for a carboxylesterase (E.C.3.1.1.1) and is polymorphic for two major electromorphs (slow and fast). Isogenic lines containing X chromosomes extracted from natural populations and substituted into a common genetic background were used to detect unlinked factors that affect the activity of the Est-6 locus. Twofold activity differences of esterase 6 (EST 6) were found among males from these derived lines, which differ only in their X chromosome. These unlinked activity modifiers identify possible regulatory elements. Immunoelectrophoresis was used to estimate quantitatively the levels of specific cross-reacting material in the derived lines. The results show that the variation in activity is due to differences in the amount of EST 6 present. The data are consistent with the hypothesis that there is at least one locus on the X chromosome that regulates the synthesis of EST 6 and that this regulatory locus may be polymorphic in natural populations.

Type
Research Article
Information
Genetics Research , Volume 43 , Issue 2 , April 1984 , pp. 181 - 190
Copyright
Copyright © Cambridge University Press 1984

References

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