Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-23T00:31:34.387Z Has data issue: false hasContentIssue false
  • English
  • Français

Some details and effects of the premeiotic controls of recombination frequencies in Neurospora crossa

Published online by Cambridge University Press:  14 April 2009

B. C. Lamb
B. C. Lamb
Affiliation:
Department of Botany, Imperial College, London, S. W. 7
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Recombination data from crosses made at a single constant temperature of incubation were compared with those from crosses transferred to a different temperature at either the time of conidiation of protoperithecia by the strain of opposite mating-type, or after fertilization when crozier stages were first visible. Results were also compared from reciprocal crosses, from crosses made in different ways and from crosses in which protoperithecia were conidiated at different stages of maturity.

Different temperature regimes during vegetative growth and proto-perithecial development had highly significant effects on subsequent meiotic recombination, while temperature differences during later premeiotic stages (between conidiation of protoperithecia and the crozier stage) had no or little effect. It was found that premeiotic controls could have as great, or greater, effects on meiotic recombination than those operating directly during meiosis. The possible adaptive significance of this is discussed.

Recombination frequencies were affected by the method of making a cross (joint-inoculation of strains of opposite mating-type, or conidiation of protoperithecia), and by protoperithecial age at the time of conidiation by the opposite mating-type. Differences in recombination between reciprocal crosses were obtained and were dependent on temperature of incubation and age of protoperithecia at the time of conidiation. Recombination was not affected by different lysine concentrations in the medium. Genetic differences in premeiotic effector-production between the strains used were inferred.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 3 , December 1971 , pp. 255 - 264
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Chauhan, K. P. S. & Abel, W. O. (1968). Evidence for the association of homologous chromosomes during premeiotic stages in Impatiens and Salvia. Chromosoma 25, 297–302.CrossRefGoogle Scholar
Frost, L. C. (1961). Heterogeneity in recombination frequencies in Neurospora crossa. Genetical Research 2, 43–62.CrossRefGoogle Scholar
Griffiths, A. J. F. (1968). Heterokaryotic transmission of factors affecting recombination in Neurospora crossa. Canadian Journal of Genetics and Cytology 10, 248–252.CrossRefGoogle Scholar
Griffiths, A. J. F. & Threlkeld, S. F. H. (1966). Internuclear effects on prototroph frequencies of some crosses in Neurospora crossa. Genetics 54, 77–87.CrossRefGoogle Scholar
Lamb, B. C. (1963). The effect of temperature on recombination frequency in Neurospora crossa. B.Sc. thesis, University of Bristol.Google Scholar
Lamb, B. C. (1966). Polarized segregation in Ascomycetes and the differential bursting of asci. Genetical Research 7, 325–334.CrossRefGoogle ScholarPubMed
Lamb, B. C. (1967). The differential maturation of asci and its relevance to recombination studies of Neurospora, Sordaria and similar Ascomycetes. Genetical Research 10, 1–12.CrossRefGoogle ScholarPubMed
Lamb, B. C. (1969 a). Related and unrelated changes in conversion and recombination frequencies with temperature in Sordaria fimicola, and their relevance to hybrid-DNA models of recombination. Genetics 62, 67–78.CrossRefGoogle ScholarPubMed
Lamb, B. C. (1969 b). Evidence from Sordaria that recombination and conversion frequencies are partly determined before meiosis, and for a general model of the control of recombination frequencies. Genetics 63, 807–820.CrossRefGoogle ScholarPubMed
Landner, L. (1970). Variation of recombination frequency in Neurospora crossa following temperature changes prior to and during meiosis and evidence for a premeiotic sensitive stage. Molecular and General Genetics 109, 219–232.CrossRefGoogle Scholar
Lawrence, C. W. (1961). The effect of radiation on chiasma formation in Tradescantia. Radiation Botany 1, 92–96.CrossRefGoogle Scholar
Lu, B. C. (1969). Genetic recombination in Coprinus. I. Its precise timing as revealed by temperature treatment experiments. Canadian Journal of Genetics and Cytology 11, 834–847.CrossRefGoogle Scholar
McNelly-Ingle, C. A., Lamb, B. C. & Frost, L. C. (1966). The effect of temperature on recombination frequency in Neurospora crossa. Genetical Research 7, 169–183.CrossRefGoogle Scholar
Mitchell, H. K. (1957). Crossing over and gene conversion in Neurospora. In Chemical Basis of Heredity (ed. McElroy, W. D. & Glass, B.), pp. 94–113. Baltimore: John Hopkins Press.Google Scholar
Phillips, R. L. (1969). Recombination in Zea mays L. II. Cytogenetic studies of recombination in reciprocal crosses. Genetics 61, 117–127.CrossRefGoogle ScholarPubMed