Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-25T06:43:50.321Z Has data issue: false hasContentIssue false

A simple and rapid technique for obtaining a high proportion of hybrid cleistothecia in Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

B. W. Bainbridge
Affiliation:
Microbiology Department, Queen Elizabeth College, London
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A technique is reported for crossing strains of Aspergillus nidulans which produces hybrid cleistothecia in 7 days. The method depends on the partial inhibition of cleistothecium formation of auxotrophic mutants by growth-factor limitation. Cleistothecia form at the junction of two strains inoculated on to complete medium. The method has a number of advantages over standard techniques.

Type
Short Papers
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Bainbridge, B. W. (1965). The arginine crossing technique. Aspergillus Newsletter 6, 89.Google Scholar
Baitnbridge, B. W. & Roper, J. A. (1966). Observations on the effects of a chromosome duplication in Aspergillus nidiulans. Journal of General Microbiology 42, 417424.CrossRefGoogle Scholar
Clutterbuck, A. J. (1973). Gene symbols in Aspergillus nidulans. Genetical Research 21, 291296.CrossRefGoogle ScholarPubMed
Cybis, J. & Weglenski, P. (1969). Effects of lysine on arginine uptake and metabolism in Aspergillus nidulans. Molecular and General Genetics 104, 282287.Google Scholar
Foley, J. M., Giles, N. H. & Roberts, C. F. (1965). Suggested modification of techniques. Aspergillus Newsletter 6, 16.Google Scholar
MacKintosh, M. E. & Pritchard, R. H. (1963). The production and replica plating of micro-colonies of Aspergillus nidulans. Genetical Research 4, 320322.Google Scholar
Pontecorvo, G., Roper, J. A., Hemmons, L. M., MacDonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advances in Genetics 5, 141238.CrossRefGoogle ScholarPubMed
Trinci, A. P. J. (1969). A kinetic study of the growth of Aspergillus nidulans and other fungi. Journal of General Microbiology 57, 1124.CrossRefGoogle ScholarPubMed