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A semi-automatic technique for conducting many bacterial matings concurrently*

Published online by Cambridge University Press:  14 April 2009

Mark Achtman
Mark Achtman
Affiliation:
Department of Molecular Biology, University of California, Berkeley
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Summary

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A technique using microserological equipment is described whereby up to 144 quantitative interrupted bacterial matings may be performed concurrently. The technique is easy to use, accurate and reproducible, and eliminates the need for agar plates. However, it is only suitable for 40–42 °C matings and mating efficiency is approximately threefold lower than in test tubes.

Type
Short Papers
Information
Genetics Research , Volume 17 , Issue 3 , June 1971 , pp. 261 - 266
Copyright
Copyright © Cambridge University Press 1971

References

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