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Selection and analysis of a mutant Paramecium tetraurelia lacking behavioural response to tetraethylammonium

Published online by Cambridge University Press:  14 April 2009

Sheng-Yung Chang  and
Ching Kung
Sheng-Yung Chang
Affiliation:
Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
Ching Kung
Affiliation:
Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
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We selected a mutant Paramecium tetraurelia which does not exhibit avoiding reaction in solutions of tetraethylammonium (TEA+), a known membrane K+-channel blocker. Behavioural reaction of the mutant to Na+ solutions was also weak. The rapid successions of avoiding reactions in Ba2+ solutions were observed in both wild type and the TEA-insensitive mutant. Formal genetic analyses showed that this mutant is due to a recessive mutation. This mutation is on a gene completely unlinked to and hypostatic in different degrees to the genes for the membrane defects of ‘pawn A’, ‘pawn B’, ‘ts-pawn C’, ‘fast-2’ and ‘paranoiac A’.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 97 - 107
Copyright
Copyright © Cambridge University Press 1976

References

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