Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-25T16:11:57.819Z Has data issue: false hasContentIssue false
  • English
  • Français

Selection and analysis of a mutant Paramecium tetraurelia lacking behavioural response to tetraethylammonium

Published online by Cambridge University Press:  14 April 2009

Sheng-Yung Chang  and
Ching Kung
Sheng-Yung Chang
Affiliation:
Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
Ching Kung
Affiliation:
Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

We selected a mutant Paramecium tetraurelia which does not exhibit avoiding reaction in solutions of tetraethylammonium (TEA+), a known membrane K+-channel blocker. Behavioural reaction of the mutant to Na+ solutions was also weak. The rapid successions of avoiding reactions in Ba2+ solutions were observed in both wild type and the TEA-insensitive mutant. Formal genetic analyses showed that this mutant is due to a recessive mutation. This mutation is on a gene completely unlinked to and hypostatic in different degrees to the genes for the membrane defects of ‘pawn A’, ‘pawn B’, ‘ts-pawn C’, ‘fast-2’ and ‘paranoiac A’.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 97 - 107
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Chang, S. Y. & Kung, C. (1973 a). Temperature-sensitive pawns: conditional behavioural mutants of Parmecium aurelia. Science 180, 1197–1199.CrossRefGoogle Scholar
Chang, S. Y. & Kung, C. (1973 b). Genetic analyses of heat-sensitive pawn mutants of Paramecium aurelia. Genetics 75, 49–59.Google Scholar
Chang, S. Y., Van Houten, J., Robles, L. J., Lui, S. S. & Kung, C. (1974). An extensive behavioural and genetic analyses of the Pawn mutants in Paramecium aurelia. Genetical Research 23, 165–173.Google Scholar
Dryl, S. (1959). Antigenic transformation in Paramecium aurelia after homologous anti-serum treatment during autogamy and conjugation. Journal of Protozoology 6, S96.Google Scholar
Eckert, R. (1972). Bioelectric control of cilia. Science 176, 473–481.Google Scholar
Friedman, K. & Eckert, R. (1973). Ionic and pharmacological modification of input resistance and excitability in Paramecium. Comparative and Biochemical physiology 45 A, 101–114.CrossRefGoogle Scholar
Kung, C. (1971 a). Genic mutations with altered system of excitation in Paramecium aurelia. I. Phenotypes of the behavioural mutants. Zeitschrift für vergleichende Physiologie 71, 142–164.Google Scholar
Kung, C. (1971 b). Genic mutations with altered system of excitation in Paramecium aurelia. II. Mutagenesis, screening and genetic analysis of the mutants. Genetics 69, 29–45.CrossRefGoogle ScholarPubMed
Kung, C. & Eckert, R. (1972). Genetic modifications of electric properties in an excitable membrane. Proceedings of the National Academy of Sciences, U.S.A. 69, 93–97.Google Scholar
Kung, C., Chang, S. Y., Satow, Y., Van Houten, J. & Hansma, H. (1975). Genetic dissection of behavior in Paramecium. Science 188, 898–904.Google Scholar
Naitoh, Y. & Eckert, R. (1968 a). Electrical properties of Paramecium caudatum: modification by bound and free cations. Zeitschrift für vergleichende Physiologie 61, 427–452.Google Scholar
Naitoh, Y. & Eckert, R. (1968 b). Electrical properties of Paramecium caudatum: all-or-none electrogenesis. Zeitschrift für vergleichende Physiologie 61, 453–472.Google Scholar
Naitoh, Y., Eckert, R. & Friedman, K. (1972). A regenerative calcium response in Paramecium. Journal of Experimental Biology. 56, 667–681.Google Scholar
Satow, Y., Chang, S. Y. & Kung, C. (1974). Membrane excitability: made temperature-dependent by mutations. Proceedings of the National Academy of Sciences, U.S.A. 71, 2703–2706.Google Scholar
Satow, Y. & Kung, C. (1974). Genetic dissection of the active electrogenesis in Paramecium aurelia. Nature 247, 69–71.CrossRefGoogle ScholarPubMed
Satow, Y. & Kung, C. (1976). A “TEA+-insensitive” mutant with increased potassium conductance in Paramecium aurelia. Journal of Experimental Biology (in the Press).CrossRefGoogle Scholar
Sonneborn, T. M. (1970). Methods in Paramecium research. In Methods of Cell Physiology, vol. 4 (ed. Prescott, D. M.), pp. 243–339. New York: Academic Press.Google Scholar
Sonneborn, T. M. (1974). Paramecium aurelia. In Handbook of Genetics, vol. 2 (ed. King, R. C.), pp. 469–594. New York: Plenum Press.CrossRefGoogle Scholar
Sonneborn, T. M. (1975). Species designations and descriptions of the 14 syngens of Paramecium aurelia. Transaction American Microscopic Society 94, 155–178.CrossRefGoogle Scholar