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Segregation of centric Y-autosome translocations in Drosophila melanogaster: I. Segregation determinants in males

Published online by Cambridge University Press:  14 April 2009

Raphael Falk ,
Shula Baker  and
Ana Rahat
Raphael Falk
Affiliation:
Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91–904, Israel
Shula Baker
Affiliation:
Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91–904, Israel
Ana Rahat
Affiliation:
Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91–904, Israel
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A special screening procedure for the detection of induced Y-autosome translocations with centric breakpoints was applied. A series of Experimental stocks was constructed, each containing a different half of one of the induced T(Y; 2)'s (T element). The three other elements that were involved in the segregation experiments in each stock were a sex chromosome (X element), an inverted chromosome 2 (A element), and a free arm of chromosome 2 (F element). It is not feasible to determine the relative frequencies of all the 16 possible gamete types by mating an Experimental stock to one tester, nor to different testers that have each at least one class of progeny of the same genotype. Each Experimental stock was mated to four different Tester stocks and the data were calibrated so that a coherent segregation pattern could be obtained.

Segregation patterns in meiosis of males from 15 Experimental stocks, each with a different T element were studied. In most Experimental stocks the T element was of the left autosomal arm, while the F element was of the right autosomal arm. In four Experimental stocks the X element segregated independently of the A, F and T elements. In these Group 1 stocks, both the F and the T elements disjoined regularly from the A element. It was concluded that the T element of these stocks had no sex-chromosome disjunction determinants (‘S-determinants’) to interact with the determinants on the X element. Both the T elements and the F elements carried autosomal disjunction determinants (‘H-determinants’) that secured the segregation of the autosomal elements. The H-determinants of the left autosomal arm were qualitatively different from those of the right arm.

In the remaining 11 Group-2 Experimental stocks the X and T elements disjoined regularly, indicating the presence of S-determinants on the T elements of these stocks. The segregation of the T and the A elements in these stocks varied from nearly complete dependence to complete independence. It was concluded that this gradation reflected differences in the quantity of H-determinants present on the T elements of these Experimental stocks. It was impossible to discriminate between a model of continuous H determinants activity and one of a finite discrete number of determinants. The results do not agree with the claim that there are no autosomal disjunction determinants in the proximal heterochromatin of chromosome 2.

The S-determinants on the BsYy+ chromosome were located both adjacent to the centromere and distally on the long arm. The latter were probably translocated to the Y chromosome together with the Bs marker.

Type
Research Article
Information
Genetics Research , Volume 45 , Issue 1 , February 1985 , pp. 51 - 79
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Appels, R. & Hilliker, A. J. (1982). The cytogenetic boundaries of the rDNA region within heterochromatin of the X-chromosome of Drosophila melanogaster and their relation to male meiotic pairing sites. Genetical Research 39, 149–156.CrossRefGoogle ScholarPubMed
Ault, J. G., Lin, H.-P. P. & Church, K. (1982). Meiosis in Drosophila melanogaster. IV. The conjunctive mechanism of the X Y bivalent. Chromosoma 86, 309–317.Google Scholar
Baker, B. S. & Carpenter, A. T. C. (1972). Genetic analysis of sexchromosomal meiotic mutants in Drosophila melanogaster. Genetics 71, 255–286.Google Scholar
Baker, B. S., Carpenter, A. T. C., Esposito, M. S., Esposito, R. E. & Sandler, L. (1976). The genetic control of meiosis. Annual Review of Genetics 10, 53–134.Google Scholar
Baker, B. S. & Hall, J. C. (1976). Meiotic mutants: genetic control of meiotic recombination and chromosome segregation. In The Genetics and Biology of Drosophila, vol. 1a (ed. Ashburner, M. and Novitski, E.), pp. 351–434. Academic Press.Google Scholar
Carpenter, A. T. C. (1972). A meiotic mutant defective in distributive disjunction in Drosophila melanogaster. Genetics 73 393–428.CrossRefGoogle Scholar
Clarke, L. & Carbon, J. (1983). Genomic substitutions of centromeres in Saccharomyces cervisiae. Nature 305, 23–28.CrossRefGoogle Scholar
Cooper, K. W. (1964). Meiotic conjunctive elements not involving chiasmata. Proceedings of the National Academy of Science of the U.S.A. 52, 1248–1255.CrossRefGoogle Scholar
Cooper, K. W. (1965). Normal spermatogenesis in Drosophila. In Biology of Drosophila (ed. Demerec, M.), pp. 1–61. New York: Hafner.Google Scholar
Debus, B. (1978). ‘Nodules’ in the achiasmatic meiosis of Bithynia (Mollusca, Prosobranchia). Chromosoma 69, 81–92.Google Scholar
Dobzhansky, Th. (1933). Studies on chromosome conjugation. II. The relation between crossing-over and disjunction of chromosomes. Zeitschrift für induktive Abstammungs- und Vererbungslehre 44, 269–309.Google Scholar
Falk, R. (1983). The effect of an unusual chromosome architecture on disjunction and non-disjunction in Drosophila. Genetical Research 41, 17–28.Google Scholar
Falk, R. & Baker, S. (1984). Production of centric-autosomal Y translocations. Drosophila Information Service 60, 104–105.Google Scholar
Falk, R., Rahat, A. & Baker, S. (1985). Segregation of centric y-autosome translocations in Drosophila melanogaster. II. Segregation determinants in females. Genetical Research 45, 81–93.CrossRefGoogle ScholarPubMed
Frost, J. N. (1961). Autosomal nondisjunction in males of Drosophila melanogaster. Genetics 46, 39–54.CrossRefGoogle ScholarPubMed
Gassner, G. (1969). Synaptonemal complexes in the achiasmatic spermatogenesis of Bolbe nigra Gigliu-Tos (Mantoidea). Chromosoma 26, 22–34.CrossRefGoogle Scholar
Gethmann, R. C. (1974). The segregational behavior of Y-2 translocations in Drosophila melanogaster. Genetics 77, s25–s26.Google Scholar
Gethmann, R. C. (1976). Meiosis in male Drosophila melanogaster II. Nonrandom segregation of compound-second chromosomes. Genetics 83, 743–751.Google Scholar
Grell, R. F. (1962). A new hypothesis on the nature and sequence of meiotic events in the female of Drosophila melanogaster. Proceedings of the National Academy of Science U.S.A. 48 165–172.Google Scholar
Grell, R. F. (1964). Chromosome size at distributive pairing in Drosophila melanogaster females. Genetics 50, 151–166.Google Scholar
Grell, R. F. (1976). Distributive pairing. In The Genetics and Biology of Drosophila, vol. 1b (ed. Ashburner, M. and Novitski, E.), pp. 435–486. Academic Press.Google Scholar
Hager, H. & Holm, D. G. (1980). Meiotic behavior of compound autosomes in females of Drosophila melanogaster: interchromosomal effects and the source of spontaneous nonsegregation. Genetics 96, 455–470.Google Scholar
Hilliker, A. J., Appels, R. & Schalet, A. (1980). The genetic analysis of Drosophila melanogaster heterochromatin. Cell 21, 607–619.Google Scholar
Hilliker, A. J. & Holm, D. G. (1975). Genetic analysis of the proximal region of chromosome-2 of Drosophila melanogaster. I. Detachment products of compound autosomes. Genetics 81, 705–721.Google Scholar
Hilliker, A. J., Holm, D. G. & Appels, R. (1982). The relationship between heterochromatic homology and meiotic segregation of compound second autosomes during spermatogenesis in Drosophila melanogaster. Genetical Research 39, 157–168.Google Scholar
Lindsley, D. L. & Grell, E. H. (1968). Genetic Variations of Drosophila melanogaster. Carnegie Institute of Washington Publication, no. 627.Google Scholar
Lindsley, D. L., Sandler, L., Baker, B. S., Carpenter, A. T. C., Denell, R. F., Hall, J. C., Jacobs, P. A., Miklos, G. L. G., Davis, B. K., Gethmann, R. C., Hardy, R. W., Hessler, A., Miller, S. M., Nozawa, H., Parry, D. M. & Gould-Somero, M. (1972). Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics 71, 157–184.CrossRefGoogle ScholarPubMed
Noda, S. (1975). Achiasmate meiosis in Fritillaria japonica group I. Different modes of bivalent formation in the two sex mother cells. Heredity 34, 373–380.Google Scholar
Novitski, E. (1976). The construction of an entire compound two chromosome. In The Genetics and Biology of Drosophila, vol. 1b (ed. Ashburner, M. and Novitski, E.), pp. 562–568. Academic Press.Google Scholar
Novitski, E. & Braver, G. (1954). An analysis of crossing over within a heterogeneous inversion in Drosophila melanogaster. Genetics 39, 197–209.CrossRefGoogle Scholar
Novitski, E., Grace, D. & Strommen, C. (1981). The entire compound autosomes of Drosophila melanogaster. Genetics 98, 257–273.Google Scholar
Rickards, G. K. (1983). Alternate-1 and alternate-2 orientations in interchange (reciprocal translocation) quadrivalents. Genetics 104, 211–213.Google Scholar
Sandler, L., Lindsley, D. L., Nicoletti, B. & Trippa, G. (1968). Mutants affecting meiosis in natural populations of Drosophila melanogaster. Genetics 60, 525–558.CrossRefGoogle ScholarPubMed
Sandler, L. & Szauter, P. (1978). The effect of recombination defective meiotic mutants on fourth-chromosome crossing over in Drosophila melanogaster. Genetics 90, 699–712.Google Scholar
Serrano, J. (1981). Male achiasmatic meiosis in Caraboidea (Coleoptera, Adephaga). Genetica 57, 131–137.Google Scholar
Welsch, B. (1973). Synaptonemal Complex und Chromosomenstruktur in der achiasmatischen Spermatogenese von Panorpa communis (Mecoptera). Chromosoma 43, 19–74.Google Scholar
Yamamoto, M. (1979). Cytological studies of heterochromatin function in the Drosophila melanogaster male: Autosomal meiotic pairing. Chromosoma 72, 293–328.Google Scholar
Yamamoto, M. & Miklos, G. L. G. (1977). Genetic dissection of heterochromatin in Drosophila: the role of basal X heterochromatin in meiotic sex chromosome behaviour. Chromosoma 60, 283–296.Google Scholar