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The satellite DNAs of Drosophila simulans

Published online by Cambridge University Press:  14 April 2009

A. R. Lohe
A. R. Lohe
Affiliation:
Molecular Biology Institute and Department of Biology, University of California, Los Angeles, California 90024
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The resolution of antibiotic-CsCl gradients enabled an examination of the satellite DNAs in the nuclear DNA of Drosophila simulans. Of the eight distinct satellite DNAs which were detected, four band at almost the same buoyant density in CsCl but can be resolved in netropsin sulphate-CsCl gradients. Each consists of a repeated sequence which, in five of the satellites, is shown to be arranged in tandem for long regions of the chromosomal DNA. One satellite (1·697 g/ml in CsCl) contains repeated sequences interspersed with other sequences. The satellite DNAs were compared with the satellite DNAs known to be present in the sibling species, D. melanogaster. The two species have different overall complements of satellite DNAs, but one satellite (1·672 g/ml) may be identical.

Type
Research Article
Information
Genetics Research , Volume 38 , Issue 3 , December 1981 , pp. 237 - 250
Copyright
Copyright © Cambridge University Press 1981

References

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