Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-20T08:41:14.079Z Has data issue: false hasContentIssue false
  • English
  • Français

Reversion studies with exrB in Escherichia coli

Published online by Cambridge University Press:  14 April 2009

Joseph Greenberg ,
Leonard Berends ,
John Donch  and
Ben Johnson
Joseph Greenberg
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Leonard Berends
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
John Donch
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Ben Johnson
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

exrB derivatives of H/r30 and WP2 are sensitive to ultraviolet radiation (UV), methylmethane sulphonate (MMS) and will not form colonies at 42 °C. Revertants to wild type were selected by plating on either nutrient plates containing MMS or nutrient plates incubated at 42 °C. Revertants selected for MMS resistance were temperature- and UV-resistant, and all revertants selected for temperature resistance were MMS and UV resistant. The frequency with which revertants appeared on MMS plates was about eight times as high as it was at 42 °C, and the variance in frequency of revertants among cultures started with small inocula was higher on selection at 42 °C than on MMS. The spontaneous frequency was estimated to be 10−6 to 10−7, sufficiently low to suggest a single mutational event. Among wild-type revertants selected at 42 °C there was a stable and an unstable class. exrB+ could be co-transduced with malB+ from stable revertants to an exrB strain, but could not from unstable revertants. None of the revertants could support the growth of amber or ochre mutants of phage T4, nor were amber and ochre suppressed exrB strains wild type with regards MMS and temperature resistance. The presence of a polarity suppressor in an exrB strain did not modify resistance to UV or temperature. Neither UV or MMS was able to induce mutations in an exrB strain.

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 2 , April 1975 , pp. 109 - 117
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Brody, S. & Yanofsky, C. (1965). Mechanism studies of suppressor-gene action. Journal of Bacteriology 90, 687–695.CrossRefGoogle ScholarPubMed
Demerec, M., Adelberg, E. A., Clark, A. J. & Hartman, P. E. (1966). A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76.CrossRefGoogle ScholarPubMed
Donch, J. J. & Greenberg, J. (1968 a). Loci of radiation sensitivity in Bs strains of Escherichia coli. Genetical Research, Cambridge 11, 183–191.CrossRefGoogle Scholar
Donch, J. & Greenberg, J. (1968 b). Ultraviolet sensitivity gene of Escherichia coli B. Journal of Bacteriology 95, 1555–1559.CrossRefGoogle ScholarPubMed
Donch, J. J. & Greenberg, J. (1974 a). The effect of lex on UV sensitivity, filament formation and λ-induction in Ion mutants of Escherichia coli. Molecular and General Genetics 128, 277–281.CrossRefGoogle ScholarPubMed
Donch, J. & Greenberg, J. (1974 b). The dominance of exrA in Escherichia coli. Molecular and General Genetics (in the Press).Google Scholar
Drake, J. W. (1970). The Molecular Basis of Mutation. San Francisco, London, Cambridge, Amsterdam: Holden-Day.Google Scholar
Fangman, W. L. & Russel, M. (1971). X-irradiation sensitivity in Escherichia coli defective in DNA replication. Molecular and General Genetics 110, 332–347.CrossRefGoogle ScholarPubMed
Greenberg, J., Berends, L. J., Donch, J. & Green, M. H. L. (1974). exrB: A malB-linked gene in Escherichia coli B involved in sensitivity to radiation and filament formation. Genetical Research, Cambridge 23, 175–184.CrossRefGoogle ScholarPubMed
Greenberg, J. & Donch, J. (1974). Sensitivity to elevated temperatures in exrB strains of Escherichia coli. Mutation Research 25, 403–405.CrossRefGoogle ScholarPubMed
Greenberg, J., Donch, J. J. & Berends, L. (1975). The dominance of exrB over exrB + in heterodiploids of Escherichia coli. Genetical Research, Cambridge 25, 39–44.CrossRefGoogle ScholarPubMed
Gundersen, W. B. (1963). New type of streptomycin resistance resulting from action of the episome-like mutator factor in Escherichia coli. Journal of Bacteriology 86, 510–516.CrossRefGoogle Scholar
Hill, R. F. (1963). The stability of spontaneous and ultraviolet-induced reversions from auxotrophs in Escherichia coli. Journal of General Microbiology 30, 289–297.CrossRefGoogle Scholar
Hill, R. F., Foulds, J., Soll, L. & Berg, P. (1969). Instability of a missense suppressor resulting from a duplication of genetic material. Journal of Molecular Biology 39, 563–581.CrossRefGoogle ScholarPubMed
Hofnung, M. (1974). Divergent operons and the genetic structure of the maltose B region in Escherichia coli K12. Genetics 76, 169–184.CrossRefGoogle ScholarPubMed
Kohiyama, M., Cousin, D., Ryter, A. & Jacob, F. (1966). Mutants thermosensibles d'Escherichia coli K12. 1. Isolement et characterisation rapide. Annals Institute Pasteur 110, 465–496.Google Scholar
Kondo, S., Ichikawa, H., Iwo, K. & Kato, T. (1970). Base-change mutagenesis and prophage induction in strains of Escherichia coli with different DNA repair capacities. Genetics 66, 187–217.CrossRefGoogle ScholarPubMed
Morse, D. E. & Primakoff, P. (1970). Relief of polarity in E. coli by ‘suA’. Nature 226, 28–31.CrossRefGoogle Scholar
Mount, D. W., Low, K. B. & Edminston, S. J. (1972). Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet light-induced mutations. Journal of Bacteriology 112, 886–893.CrossRefGoogle Scholar
Mount, D. W., Walker, A. C. & Kosel, C. (1973). Suppression of lex mutations affecting deoxyribonucleic acid repair in Escherichia coli K-12 by closely linked thermosensitive mutations. Journal of Bacteriology 116, 950–956.CrossRefGoogle Scholar
Schwartz, N. M. (1967 a). Revertant instability in Escherichia coli. I. Mutagen and allele specificity. Genetics 57, 495–503.CrossRefGoogle ScholarPubMed
Schwartz, N. M. (1967 b). Revertant instability in Escherichia coli. II. Genetic studies. Genetics 57, 505–512.CrossRefGoogle ScholarPubMed
Wechsler, J. A. & Gross, J. D. (1971). Escherichia coli mutants temperature-sensitive for DNA synthesis. Molecular and General Genetics 113, 273–284.CrossRefGoogle ScholarPubMed
Wechsler, J. A., Nüsslein, V., Otto, B., Klein, A., Bonhoeffer, F., Herrmann, R., Gloger, L., & Schaller, H. (1973). Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication. Journal of Bacteriology 113, 1381–1388.CrossRefGoogle ScholarPubMed
Witkin, E. M. (1969). Ultraviolet-induced mutation and DNA repair. Annual Review of Microbiology 23, 487–514.CrossRefGoogle ScholarPubMed