Hostname: page-component-848d4c4894-jbqgn Total loading time: 0 Render date: 2024-07-05T11:10:27.014Z Has data issue: false hasContentIssue false
  • English
  • Français

Reversion analysis of [psi−] mutations in Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

P. M. Lund  and
B. S. Cox
P. M. Lund
Affiliation:
Botany School, South Parks Road, Oxford, U.K.
B. S. Cox
Affiliation:
Botany School, South Parks Road, Oxford, U.K.

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Mutants of [psi] a cytoplasmically inherited factor in the yeast Saccharomyces cerevisiae were isolated after treatment with a variety of agents including conventional mutagens and a number of compounds which cause loss of [psi] at high frequencies, namely methanol, KCl, dimethyl sulphoxide and guanidine hydrochloride. In [psi−] mutants the suppressor SUQ5 does not suppress ochre mutations such as ade.2.1.

Reversion analysis of the [psi−] mutants revealed three classes: (1) a class of agents producing [psi−] mutations which could readily revert to [psi+] (methanol, KCl and dimethyl sulphoxide belong to this class), (2) those which could not be shown to revert (GuHCl) and (3) the conventional mutagens which produced both revertible and apparently non-revertible [psi−] mutations. We conclude that GuHCl causes a deletion or loss of the [psi] factor. Methanol may cause an alteration of ‘state’ for example, of a promoter, and KCl may be selecting or inducing low copy number variants of [psi+] strains. It is possible that DMSO may be involved in regulation of [psi].

Type
Research Article
Information
Genetics Research , Volume 37 , Issue 2 , April 1981 , pp. 173 - 182
Copyright
Copyright © Cambridge University Press 1981

References

REFERENCES

Conde, J. & Fink, G. R. (1976). A mutant of Saccharomyces cerevisiae defective for nuclear fusion. Proceedings of the National Academy of Science of the United States of America 73, 3651–3655.CrossRefGoogle ScholarPubMed
Cox, B. S. (1965). ψ, a cytoplasmic suppressor of super-suppressor in yeast. Heredity 20, 505–521.CrossRefGoogle Scholar
Cox, B. S. & Bevan, E. A. (1962). Aneuploidy in yeast. New Phytologist 61, 342–355.CrossRefGoogle Scholar
Cox, B. S., Tuite, M. F. & Mundy, C. J. (1980). Reversion from suppression to non-suppression in SUQ5 [psi +] strains of yeast: the classification of mutations. Genetics 95, 589–609.CrossRefGoogle Scholar
Friend, C., Scher, W., Holland, J. G. & Sato, T. (1971). Hemoglobin synthesis in marine virus-induced leukemia cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proceedings of the National Academy of Sciences of the United States of America 65, 378–382.CrossRefGoogle Scholar
Juliani, M. H., Gambarini, A. G. & Costa, S. O. P. (1975 a). Induction of ρ− mutants in Saccharomyces cerevisiae by guanidine hydrochloride. I. Genetic analysis. Mutation Research 29, 67–75.Google Scholar
Juliani, M. H. & Costa, S. O. P. (1975 b). Induction of ρ− mutants inSaccharomyces cerevisiae by guanidine hydrochloride. II. Conditions that prevent ρ− induction. Mutation Research 30, 335–342.CrossRefGoogle Scholar
Liebman, S. W., Stewart, J. W. & Sherman, F. (1975). Serine substitutions caused by an ochre suppressor in yeast. Journal of Molecular Biology 94, 595–610.CrossRefGoogle ScholarPubMed
Lindegren, G., Hwang, Y. L., Oshima, Y. & Lindegren, C. C. (1965). Genetical mutants induced by ethyl mthanesulfonate in Saccharomyces. Canadian Journal of Genetics and Cytology 7, 491–499.CrossRefGoogle ScholarPubMed
Mundy, C. R. (1979). The genetics of translation in yeast. D.Phil, thesis, Oxford University, Oxford, U.K.Google Scholar
Nakanishi, S., Adhya, S., Gottesman, M. & Pastan, I. (1974). Effects of dimethylsulfoxide on the E. coli gal operon and on bacteriophage lambda in vivo. Cell 3, 39–46.CrossRefGoogle Scholar
Oliver, S. G. & Williamson, D. H. (1977). Mutants of yeast specifically resistant to petite induction by fluorinated pyrimidines. Biochemical Genetics 15, 775–783.CrossRefGoogle ScholarPubMed
Rasse-Messenguy, F. & Fink, G. R. (1973). Temperature-sensitive nonsense suppressors in yeast. Genetics 75, 459–464.Google Scholar
Tuite, M. F. (1978). Genetics of nonsense suppressors in yeast. D.Phil, thesis, Oxford University, Oxford, U.K.Google Scholar
Tuite, M. F. & Cox, B. S. (1980). Ultraviolet mutagenesis studies of [p¯si], a cytoplasmic determinant of Saccharomyces cerevisiae. Genetics. 95, 611–630.CrossRefGoogle ScholarPubMed
Tuite, M. F., Mundy, C. J. & Cox, B. S. (1980). A study of some non-mutagenic substances that cause reversion from [p¯si+] to [p¯si−] in Saccharomyces cerevisiae. Genetics. (In the Press.)Google Scholar
Young, C. S. H. & Cox, B. S. (1972). Extrachromosomal elements in a super-suppression system of yeast. II. Relations with other extrachromosomal elements. Heredity 28, 189–199.CrossRefGoogle Scholar
Young, C. S. H. & Cox, B. S. (1975). Extrachromosomal elements in a super-suppression system of yeast. III. Enhanced detection of weak suppressors in certain non-suppressed strains. Heredity 34, 83–92.CrossRefGoogle Scholar