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Reversion analysis of [psi−] mutations in Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

P. M. Lund  and
B. S. Cox
P. M. Lund
Affiliation:
Botany School, South Parks Road, Oxford, U.K.
B. S. Cox
Affiliation:
Botany School, South Parks Road, Oxford, U.K.
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Mutants of [psi] a cytoplasmically inherited factor in the yeast Saccharomyces cerevisiae were isolated after treatment with a variety of agents including conventional mutagens and a number of compounds which cause loss of [psi] at high frequencies, namely methanol, KCl, dimethyl sulphoxide and guanidine hydrochloride. In [psi−] mutants the suppressor SUQ5 does not suppress ochre mutations such as ade.2.1.

Reversion analysis of the [psi−] mutants revealed three classes: (1) a class of agents producing [psi−] mutations which could readily revert to [psi+] (methanol, KCl and dimethyl sulphoxide belong to this class), (2) those which could not be shown to revert (GuHCl) and (3) the conventional mutagens which produced both revertible and apparently non-revertible [psi−] mutations. We conclude that GuHCl causes a deletion or loss of the [psi] factor. Methanol may cause an alteration of ‘state’ for example, of a promoter, and KCl may be selecting or inducing low copy number variants of [psi+] strains. It is possible that DMSO may be involved in regulation of [psi].

Type
Research Article
Information
Genetics Research , Volume 37 , Issue 2 , April 1981 , pp. 173 - 182
Copyright
Copyright © Cambridge University Press 1981

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