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Resistance to p-fluorophenylalanine in diploid/haploid dikaryons: dominance modifier gene explained as a controller of hybrid multimer formation

Published online by Cambridge University Press:  14 April 2009

D. Lewis  and
Dina Vakeria
D. Lewis
Affiliation:
Department of Botany & Microbiology, University College London, Gower Street, London WCJE 6BT
Dina Vakeria
Affiliation:
Department of Botany & Microbiology, University College London, Gower Street, London WCJE 6BT
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Summary

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The gene pfpr-10 in Coprinus confers resistance to p-fluorophenylalanine. The resistance of the heterozygote pfpr-10/pfp8-10 is completely recessive, both in diploids and dikaryons. A dominance modifier Mod+ makes pfpr-10 dominant in a dikaryon but not in a cliploid. It also enhances the degree of resistance in r homozygotes. These results are explained on a hypothetical model based upon a hexameric product of the pfpr-10 gene, with resistance to PFP being proportional to the percentage of homo r hexamers. The Mod+ is presumed to act by keeping the r and s products separate so that hybrid multimers are reduced to a minimum. Critical threshold concentrations of PFP for diploid/haploid dikaryons in 40 different combinations of pfpr and Mod+ genes cover a wide range of gene doses and percentage of homo r multimers. The relationship between the critical threshold concentrations and the calculated percentage of homo r multimers supports the model.

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 1 , August 1977 , pp. 31 - 43
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Casselton, L. A. & Lewis, D. (1966). Compatibility and stability of diploids in Coprinus lagopus. Genetical Research 8. 61–72.CrossRefGoogle ScholarPubMed
Hiwatashi, K. & Myohara, K. (1976). A modifier gene involved in the expression of the dominant mating type allele in Paramecium caudatum. Qenetical Research 27. 135–141.Google ScholarPubMed
Lewis, D. (1961). Genetical analysis of methionine suppressors in Coprinus. Genetical Research 2. 141–155.CrossRefGoogle Scholar
Lewis, D. & North, J. C. (1974). Linkage maps of Coprinus lagopus. Handbook of Microbiology vol. iv. Cleveland: CRC Press Inc.Google Scholar
North, J. C. (1977). The effects of griseofulvin on diploid strains of Coprinus lagopus. Journal of General Microbiology 98. 529–534.CrossRefGoogle Scholar
Senathirajah, S. & Lewis, D. (1975). Reistance to amino acid analogues in Coprinus: dominance modifier genes and dominance reversal in dikaryons and diploids. Genetical Research 25. 95–107.CrossRefGoogle Scholar
Müller-Hill, B. (1975). Lac repressor and lac operator. Progress in Biophysics & Molecular Biology 30. 227–252.CrossRefGoogle ScholarPubMed