Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T08:33:02.572Z Has data issue: false hasContentIssue false
  • English
  • Français

Repair of double-strand breaks and lethal damage in DNA of Ustilago maydis

Published online by Cambridge University Press:  14 April 2009

S. Leaper ,
M. A. Resnick  and
R. Holliday
S. Leaper
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
M. A. Resnick
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
R. Holliday
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The size of nuclear DNA from wild-type Ustilago maydis was determined to be approximately 6·09 ± 0·3 × 108 daltons from neutral sucrose gradient sedimentation analysis. Following exposure to ionizing radiation the nuclear DNA size was reduced due to the production of double-strand breaks in the DNA. These breaks were repaired when the irradiated cells were incubated in medium for at least one hour after irradiation. The repair was seen as a shift in the DNA profile from a low molecular weight region where the control DNA sedimented. Inhibition of protein synthesis by cycloheximide prevented this type of repair. Blocking protein synthesis also decreased the survival of irradiated wild-type cells but not radiation-sensitive mutants. Protein synthesis was necessary within the first one and a half hours after irradiation for the survival of wild-type cells to be unaffected. The results provide additional evidence for an inducible repair process in U. maydis.

Type
Research Article
Information
Genetics Research , Volume 35 , Issue 3 , June 1980 , pp. 291 - 307
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Banks, G. R. (1973). Mitochondrial DNA synthesis in permeable cells. Nature New Biology 245, 196–199.CrossRefGoogle ScholarPubMed
Blamire, J., Cryer, D. R., Finkelstein, B. & Marmur, J. (1972). Sedimentation properties of yeast nuclear and mitochondrial DNA. Journal of Molecular Biology 67, 11–24.CrossRefGoogle ScholarPubMed
Bonura, T., Town, C. D., Smith, K. C. & Kaplan, H. S. (1975). The influence of oxygen on the yield of DNA double-strand breaks in X-irradiated Escherichia coli K12. Radiation Research 63, 567–577.CrossRefGoogle Scholar
Burgi, E. & Hershey, A. D. (1963). Sedimentation rate as a measure of molecular weight of DNA. Biophysics Journal 3, 309–321.CrossRefGoogle ScholarPubMed
Burrell, A. D., Feldschreiber, P. & Dean, C. J. (1971). DNA membrane association and the repair of double breaks in X-irradiated Micrococcus radiodurans. Biochemica et Biophysica Acta 247, 38–53.CrossRefGoogle ScholarPubMed
Corry, P. M. & Cole, A. (1973). Double strand rejoining in mammalian DNA. Nature New Biology 245, 100–101.Google ScholarPubMed
Duell, E. A., Inoue, S. & Utter, M. F. (1964). Isolation and properties of intact mitochondria from sphaeroplasts of yeast. Journal of Bacteriology 88, 1762–1773.CrossRefGoogle Scholar
Ehmann, U. K. & Lett, J. T. (1973). Review and evaluation of molecular weight calculations from the sedimentation profiles of irradiated DNA. Radiation Research 54, 152–162.CrossRefGoogle ScholarPubMed
Friefelder, D. (1965). Mechanism of inactivation of coliphage T7 by X-rays. Proceedings of the National Academy of Sciences of the U.S.A. 54, 128–134.CrossRefGoogle Scholar
Friefelder, D. (1970). Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. Journal of Molecular Biology 54, 567–577.Google Scholar
Hariharan, P. V. & Hutchinson, F. (1973). Neutral sucrose gradient sedimentation of very large DNA from Bacillus subtilis. II. Double-strand breaks formed by γ-ray irradiation of cells. Journal of Molecular Biology 75, 479–494.CrossRefGoogle Scholar
Holliday, R. (1961 a). The genetics of Ustilago maydis. Genetical Research, Cambridge 2, 204–230.CrossRefGoogle Scholar
Holliday, R. (1961 b). Induced mitotic crossing-over in Ustilago maydis. Genetical Research, Cambridge 2, 231–248.CrossRefGoogle Scholar
Holliday, R. (1965). Radiation sensitive mutants of Ustilago maydis. Mutation Research 2, 557–559.CrossRefGoogle ScholarPubMed
Holliday, R. (1967). Altered recombination frequencies in radiation sensitive strains of Uslilago. Mutation Research 4, 275–288.CrossRefGoogle Scholar
Holliday, R. (1971). Biochemical measure of the time and frequency of radiation induced allelic recombination in Ustilago. Nature New Biology 232, 233–236.CrossRefGoogle ScholarPubMed
Holliday, R. (1974). Ustilago maydis. In Handbook of Genetics, vol. 1 (ed. King, R. C.), pp. 575–595. New York: Plenum Press.Google Scholar
Holliday, R. (1975). Further evidence for an inducible recombination repair system in Ustilago maydis. Mutation Research 29, 149–153.CrossRefGoogle ScholarPubMed
Holliday, R., Halliwell, R. E., Evans, M. W. & Rowell, V. (1976). Genetic characterization of rec–1, a mutant of Ustilago maydis defective in repair and recombination. Genetical Research, Cambridge 27, 413–453.CrossRefGoogle ScholarPubMed
Kaplan, H. S. (1966). DNA-strand soisson and loss of viability after X-irradiation of normal and sensitized bacterial cells. Proceedings of the National Academy of Sciences of the U.S.A. 55, 1442–1446.CrossRefGoogle Scholar
Kerridge, D. (1958). The effect of actidione and other antifungal agents on nucleic acid and protein synthesis in Saccharomyces carlbergensis. Journal of General Microbiology 19, 497–506.CrossRefGoogle Scholar
Kitayama, S. & Matsuyama, A. (1971). Double-strand scissons in DNA of γ-irradiated Micrococcus radiodurans and their repair during post-irradiation incubation. Agricultural & Biological Chemistry 35, 644–652.CrossRefGoogle Scholar
Krasin, F. & Hutchinson, F. (1977). Repair of DNA double-strand breaks in Escherichia coli which require recA function and the presence of a duplicate genome. Journal of Molecular Biology 116, 81–98.CrossRefGoogle ScholarPubMed
Kutter, E. M. & Wiberg, J. S. (1968). Degradation of cytosine-containing bacterial and bacteriophage DNA after infection of Escherichia coli B with bacteriophage T4 D wild-type and with mutants defective in genes 46, 47 and 56. Journal of Molecular Biology 38, 395–411.CrossRefGoogle Scholar
Lange, C. S. (1974). The organization and repair of mammalian DNA. FEBS Letters 44, 153–156.CrossRefGoogle ScholarPubMed
Lehmann, A. R. & Stevens, S. (1977). The production and repair of double strand breaks in cells from normal humans and from patients with ataxia telangiectasia. Biochemica et Biophysica Acta 474, 49–60.CrossRefGoogle Scholar
Leighton, B. & Rubenstein, I. (1969). Calibration of molecular weightscales for DNA. Journal of Molecular Biology 46, 313–328.CrossRefGoogle Scholar
Petes, T. D. & Fangman, W. L. (1972). Sedimentation properties of yeast chromosomal DNA. Proceedings of the National Academy of Sciences of the U.S.A. 69, 1188–1191.CrossRefGoogle ScholarPubMed
Randolph, M. L. & Setlow, U. K. (1972). Mechanism of inactivation of transforming deoxyribonucleic acid by X-rays. Journal of Bacteriology 106, 221–226.CrossRefGoogle Scholar
Resnick, M. A. (1976). The repair of double-strand breaks in DNA: a model involving recombination. Journal of Theoretical Biology 59, 97–106.CrossRefGoogle Scholar
Resnick, M. A. (1978 a). The induction of molecular and genetic recombination in eukaryotic cells. Advances in Radiation Biology (In the Press.)CrossRefGoogle Scholar
Resnick, M. A. (1978 b). The importance of DNA double-strand break repair in yeast. In DNA Repair Mechamisms (ed. Hanawalt, P. C., Friedberg, E. C. & Fox, C. F.), New York: Academic Press. (In the Press.)Google Scholar
Resnick, M. A. & Holliday, R. (1971). Genetic repair and the synthesis of nitrate reductase in Ustilago maydis after UV irradiation. Molecular and General Genetics 111, 171–184.CrossRefGoogle Scholar
Resnick, M. A. & Martin, P. (1976). The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control. Molecular and General Genetics 143, 119–129.CrossRefGoogle ScholarPubMed
Unrau, P. (1975). The excision of pyrimidine dimers from the DNA of mutant and wild-type strains of Ustilago. Mutation Research 29, 53–65.CrossRefGoogle ScholarPubMed
Zimm, B. H. (1974). Anomalies in sedimentation. IV. Decrease in sedimentation coefficients of chains at high fields. Biophysical Chemistry 1, 279–291.CrossRefGoogle ScholarPubMed