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Repair of double-strand breaks and lethal damage in DNA of Ustilago maydis

Published online by Cambridge University Press:  14 April 2009

S. Leaper ,
M. A. Resnick  and
R. Holliday
S. Leaper
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
M. A. Resnick
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
R. Holliday
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA

Summary

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The size of nuclear DNA from wild-type Ustilago maydis was determined to be approximately 6·09 ± 0·3 × 108 daltons from neutral sucrose gradient sedimentation analysis. Following exposure to ionizing radiation the nuclear DNA size was reduced due to the production of double-strand breaks in the DNA. These breaks were repaired when the irradiated cells were incubated in medium for at least one hour after irradiation. The repair was seen as a shift in the DNA profile from a low molecular weight region where the control DNA sedimented. Inhibition of protein synthesis by cycloheximide prevented this type of repair. Blocking protein synthesis also decreased the survival of irradiated wild-type cells but not radiation-sensitive mutants. Protein synthesis was necessary within the first one and a half hours after irradiation for the survival of wild-type cells to be unaffected. The results provide additional evidence for an inducible repair process in U. maydis.

Type
Research Article
Information
Genetics Research , Volume 35 , Issue 3 , June 1980 , pp. 291 - 307
Copyright
Copyright © Cambridge University Press 1980

References

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