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Recombination within the Y locus in Ascobolus immersus

Published online by Cambridge University Press:  14 April 2009

A. Kruszewska  and
W. Gajewski
A. Kruszewska
Affiliation:
Department of General Genetics, Polish Academy of Sciences, Warsaw, Poland
W. Gajewski
Affiliation:
Department of General Genetics, Polish Academy of Sciences, Warsaw, Poland
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Mutants of the Y locus differed appreciably in their basic conversion frequencies (frequencies of conversion in one-point crosses) to wild type. The differences in the basic conversion frequencies in the opposite direction, i.e. from corresponding wild-type allele to mutant, were in general not pronounced. For some alleles frequencies of conversion in both directions were similar, but for the others they differed markedly. No evident correlation between the position of mutants on the map and their basic conversion frequencies was observed.

In two-point crosses in repulsion, the great majority of recombinant octads were of conversion type. In these crosses symmetry or asymmetry of conversion depended mainly on similarity or differences in basic conversion frequencies of mutants crossed. In crosses between mutants from different clusters the recombination frequencies were near to the sums of their basic conversion frequencies. Such ‘mutant specificity’ makes it impossible to establish the linear order of mutants on the basis of recombination frequencies in two-point crosses.

The results of two-point crosses in repulsion between mutants within clusters pointed to the influence of one allele on the frequency of conversion of another one. This ‘marker effect’ was also evident in some three-point crosses.

The frequencies of simultaneous conversions in two-point crosses in coupling did not show negative correlation with the distances between the mutants involved.

It seems that many of the data presented here are most easily explained by recently developed hybrid DNA models.

Type
Research Article
Information
Genetics Research , Volume 9 , Issue 2 , April 1967 , pp. 159 - 177
Copyright
Copyright © Cambridge University Press 1967

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