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Recombination between the X and Y chromosomes and the Sxr region of the mouse

Published online by Cambridge University Press:  14 April 2009

Anne McLaren ,
Elizabeth Simpson ,
Colin E. Bishop ,
Michael J. Mitchell  and
Susan M. Darling
Anne McLaren*
Affiliation:
MRC Mammalian Development Unit, Wolfson House, 4 Stephenson Way, London NW1 2HE
Elizabeth Simpson
Affiliation:
Transplantation Biology Section, Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ
Colin E. Bishop
Affiliation:
Molecular Genetics Research Laboratory, Department of Obstetrics and Gynecology, Division of Reproductive Genetics, University of Tennessee, Memphis, TN 38105.
Michael J. Mitchell
Affiliation:
Molecular Genetics Research Laboratory, Department of Obstetrics and Gynecology, Division of Reproductive Genetics, University of Tennessee, Memphis, TN 38105.
Susan M. Darling
Affiliation:
MRC Mammalian Development Unit, Wolfson House, 4 Stephenson Way, London NW1 2HE
*
*Corresponding author.
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Summary

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The Sxr (sex-reversed) region that carries a copy of the mouse Y chromosomal testis-determining gene can be attached to the distal end of either the Y or the X chromosome. During male meiosis, Sxr recombined freely between the X and Y chromosomes, with an estimated recombination frequency not significantly different from 50% in either direction. During female meiosis, Sxr recombined freely between the X chromosome to which it was attached and an X-autosome translocation. A male mouse carrying the original Sxra region on its Y chromosome, and the shorter Sxrb variant on the X, also showed 50% recombination between the sex chromosomes. Evidence of unequal crossing-over between the two Sxr regions was obtained: using five markers deleted from Sxrb, 3 variant Sxr regions were detected in 159 progeny (1·9%). Four other variants (one from the original cross and three from later generations) were presumed to have been derived from illegitimate pairing and crossing-over between Sxrb and the homologous region on the short arm of the Y chromosome. The generation of new variants throws light on the arrangement of gene loci and other markers within the short arm of the mouse Y chromosome.

Type
Research Article
Information
Genetics Research , Volume 60 , Issue 3 , December 1992 , pp. 175 - 184
Copyright
Copyright © Cambridge University Press 1992

References

Bishop, C. E., Boursot, P., Baron, B., Bonhomme, F. & Hatat, D. (1985). Most classical Mus musculus domesticus laboratory mouse strains carry a Mus musculus musculus Y chromosome. Nature 315, 70–72.CrossRefGoogle ScholarPubMed
Bishop, C. E., Weith, A., Mattei, M. G. & Roberts, C. (1988). Molecular aspects of sex determination in mice: an alternative model for the origin of the Sxr region. In Sex Determination in Mouse and Man (ed. McLaren, A. and Ferguson-Smith, M. A.), pp. 119–124. Royal Society, London.Google Scholar
Burgoyne, P. S. (1982). Genetic homolgy and crossing over in the X and Y chromosomes of mammals. Human Genetics 61, 85–90.CrossRefGoogle Scholar
Burgoyne, P. S., Levy, E. R. & McLaren, A. (1986). Spermatogenic failure in male mice lacking H-Y antigen. Nature 320, 170–172.CrossRefGoogle ScholarPubMed
Cattanach, B. M., Evans, E. P., Burtenshaw, M. D. & Barlow, J. (1982). Male, female and intersex development in mice of identical chromosome constitution. Nature 300, 445–446.CrossRefGoogle ScholarPubMed
Cattanach, B. M., Pollard, C. E. & Hawkes, S. G. (1971). Sex-reversed mice: XX and XO males. Cytogenetics 10, 318–337.CrossRefGoogle ScholarPubMed
Cattanach, B. M., Rasberry, C., Burtenshaw, M. D. & Evans, E. P. (1990). Illegitimate pairing of the X and Y chromosomes in Sxr mice. Genetical Research 56, 121–128.CrossRefGoogle ScholarPubMed
Chandley, A. C. & Speed, R. M. (1987). Cytological evidence that the Sxr fragment of XY, Sxr mice pairs homologously at meiotic prophase with the proximal testis-determining region. Chromosoma 95, 345–349.CrossRefGoogle ScholarPubMed
Evans, E. P. & Phillips, R. J. S. (1975). Inversion heterozygosity and the origin of XO daughters of Bpa/ + female mice. Nature 256, 40–41.CrossRefGoogle ScholarPubMed
Epplen, J. T., Studer, R. & McLaren, A. (1988). Heterogeneity in the Sxr (sex-reversal) locus of the mouse as revealed by synthetic probes. Genetical Research 51, 239–246.CrossRefGoogle Scholar
Falconer, D. (1953). Total sex-linkage in the house mouse. Zeitschift für induktive Abstammungs-und Vererbungslehre 85, 210–219.Google ScholarPubMed
Gubbay, J., Koopman, P., Collignon, J., Burgoyne, P. & Lovell-Badge, R. (1990 a). Normal structure and expression of Zfy genes in XY female mice mutant in Tdy. Development 109, 647–653.CrossRefGoogle ScholarPubMed
Gubbay, J., Collignon, J., Koopman, P., Capel, B., Economou, A., Munsterberg, A., Vivian, N., Goodfellow, P. & Lovell-Badge, R. (1990 b). A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes. Nature 346, 245–250.CrossRefGoogle Scholar
Harbers, K., Soriano, P., Muller, U. & Jaenisch, R. (1986). High frequency of unequal recombination in pseudo autosomal region shown by proviral insertion in transgenic mouse. Nature 324, 682–685.CrossRefGoogle Scholar
McLaren, A. (1986). Sex ratio and testis size in mice carrying Sxr and T(X; 16)16H. Developmental Genetics 7, 177–185.CrossRefGoogle Scholar
McLaren, A. & Burgoyne, P. S. (1983). Daughterless X Sxr/Y Sxr mice. Genetical Research 42, 345–349.CrossRefGoogle ScholarPubMed
McLaren, A., Simpson, E., Epplen, J. T., Studer, R., Koopman, P., Evans, E. P. & Burgoyne, P. S. (1988). Location of the genes controlling H-Y antigen expression and testis determination on the mouse Y chromosome. Proceedings of the National Academy of Sciences, USA 85, 6442–6445.CrossRefGoogle ScholarPubMed
McLaren, A. & Monk, M. (1982). Fertile females produced by inactivation of an X chromosome of sex-reversed mice. Nature 300, 446–448.CrossRefGoogle Scholar
McLaren, A., Simpson, E., Tomonari, K., Chandler, P. & Hogg, H. (1984). Male sexual differentiation in mice lacking H-Y antigen. Nature 312, 552–555.CrossRefGoogle ScholarPubMed
Mardon, G., Mosher, R., Disteche, C. M., Nishioka, Y., McLaren, A. & Page, D. C. (1989). Duplication, deletion and polymorphism in the sex-determining region of the mouse Y chromosome. Science 243, 78–80.CrossRefGoogle ScholarPubMed
Mitchell, M. J., Woods, D. R., Tucker, P. K., Opp, J. S. & Bishop, C. E. (1991). Homology of a candidate spermatogenic gene to the ubiquitin activating enzyme El. Nature 354, 483–486.CrossRefGoogle Scholar
Mitchell, M. J. & Bishop, C. E. (1992). A structural analysis of the Sxr region of the mouse Y chromosome. Genomics 12, 26–34.CrossRefGoogle ScholarPubMed
Roberts, C., Weith, A., Passage, E., Michet, J. L., Mattei, M. G. & Bishop, C. E. (1988). Molecular and cytogenetic evidence for the location of Tdy and Hya on the mouse Y chromosome short arm. Proceedings of the National Academy of Sciences USA 85, 6646–6649.CrossRefGoogle ScholarPubMed
Simpson, E., McLaren, A., Chandler, P. & Tomonari, K. (1984). Expression of H-Y antigen by female mice carrying Sxr. Transplantation 37, 17–21.CrossRefGoogle ScholarPubMed
Simpson, E., Chandler, P., Tomonari, K., Loveland, B. & McLaren, A. (1986). T-cell and antibody typing of a mouse population segregating for Sxr and H-2 haplotype. Cellular Immunology 98, 46–56.CrossRefGoogle ScholarPubMed
Simpson, E. M. –Page, D. C. (1991). An interstitial deletion in mouse Y chromosomal DNA created a transcribed Zfy fusion gene. Genomics 11, 601–608.CrossRefGoogle ScholarPubMed
Sutcliffe, M. J. & Burgoyne, P. S. (1989). Analysis of the testes of H-Y negative XO Sxrb mice suggests that the spermatogenesis gene (Spy) acts during the differentiation of the A spermatogonia. Development 107, 373–380.CrossRefGoogle Scholar