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Recombination and complementation between R factors in Escherichia coli K 12

Published online by Cambridge University Press:  14 April 2009

T. J. Foster  and
T. G. B. Howe
T. J. Foster
Affiliation:
Department of Bacteriology, The Medical School, University Walk, Bristol BS8 1TD
T. G. B. Howe
Affiliation:
Department of Bacteriology, The Medical School, University Walk, Bristol BS8 1TD
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Recombination between chloramphenicol-sensitive (Cms) mutants of Rl, and R100, has been demonstrated in Escherichia coli K12 rec+; it occurs at reduced frequency in recB and recC, and is not detectable in reeA, indicating that R factor recombination depends on host functions. Some mutants of R1 also recombine with an R100 mutant in a similar way. recA cells carrying an R1 and an R100 Cms mutant (hetero-R state) have a low level of chloramphenicol-resistance, and form a chloramphenicol acetyl transferase that has lower specific activity than enzyme from hosts carrying wild-type or recombinant factors. These results suggest the occurrence of interallelic complementation between mutant R factors.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 3 , December 1971 , pp. 287 - 297
Copyright
Copyright © Cambridge University Press 1971

References

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