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Radiation-induced alcohol dehydrogenase mutants in maize following allyl alcohol selection of pollen

Published online by Cambridge University Press:  14 April 2009

Michael Freeling  and
David S. K. Cheng
Michael Freeling
Affiliation:
Genetics Department, University of California, Berkeley, Berkeley, California 94720
David S. K. Cheng
Affiliation:
Genetics Department, University of California, Berkeley, Berkeley, California 94720

Summary

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The alcohol dehydrogenase-1 gene in maize presents advantages for mutational analysis. Foremost among these is the ability to chemically select ADH-negative and ADH-low gametophytes owing to their resistance to allyl alcohol vapour. Immature tassels were irradiated with either 220 kV X-rays or 400 MeV/amu accelerated neon-ions; spontaneous mutants were also selected and recovered. RBE for neon-20 was about 5. A total of 70 presumptive mutants were placed into one of four classes on the basis of allozyme profiles following electrophoresis and ADH staining: (A) dysfunction, (B) underproducer, (C) overproducer, and (D) up-Adh2 gene. Mutants have been recovered and confirmed in the first three classes. These include two male-transmissible deletion-type lesions induced by X-rays, five underproducer transpositions and one overproducer transposition induced by neon-20. Certain of the neoninduced alleles are unstable in their expression. All 70 mutants are chromosomal aberrations; no intragenic lesions were recovered although our experimental design would have preferentially recovered them if they had occurred.

The Discussion considers the mutagenic action of ionizing radiation, and especially the well-documented differences between maize and Drosophila data. In particular, the effect of these chromosome derangements on the ‘programmable’ component(s) of the Adhl cistron is discussed.

Type
Research Article
Information
Genetics Research , Volume 31 , Issue 2 , April 1978 , pp. 107 - 129
Copyright
Copyright © Cambridge University Press 1978

References

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