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Plasmid-mediated transmission of chromosomal genes in Pseudomonas glycinea*

Published online by Cambridge University Press:  14 April 2009

George H. Lacy
Affiliation:
Department of Plant Pathology, University of California, Riverside, California 92502, U.S.A.
John V. Leary
Affiliation:
Department of Plant Pathology, University of California, Riverside, California 92502, U.S.A.
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Summary

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Complementing diauxotrophic mutants of P. glycinea were mated in combination in which one or both mutants contained a resistance plasmid. The transfer of chromosmal markers was scored by the appearance of prototrophy at one or more of the auxotrophic loci. The resistance plasmid RP1 was equally or slightly more efficient than R6886 in the transmission of chromosomal genes.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1976

References

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