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Physiological genetics of melanotic tumours in Drosophila melanogaster VII. The relationship of dietary sterols to tumour penetrance

Published online by Cambridge University Press:  14 April 2009

Jonathan Cooke  and
J. H. Sang
Jonathan Cooke
Affiliation:
School of Biology, Sussex University, Brighton, England
J. H. Sang
Affiliation:
School of Biology, Sussex University, Brighton, England
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Summary

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A strain of Drosophila, homozygous for the variably penetrant gene tu bw, which causes the formation of abnormal masses of melanizing haemocytes (melanotic tumours), has been reared on defined axenic diets containing various sterols, both singly and in pairs. Both optimal and deficient nutritional levels of sterol have been employed, as well as certain sterols inadequate by themselves to support development. The effect of these diets upon probability of tumour formation has been studied in relation to their nutritional adequacy, as defined by the growth-rate, survival, and in one case, adult body-weight.

The results demonstrate a rather complex pattern of interaction between dietary sterols in determining the variables of the phenotype produced, under circumstances suggesting that all the sterols investigated have entered the developing larvae. There is only a partial overall correlation, and occasionally an inverse relationship, between tumour suppressant and growth-promoting properties of particular sterols. Within single molecules, structural sterol features tend to exert their characteristic effects additively upon the phenotype, except for an inadequacy in utilization of molecules containing the Δ7 double bond, which dominates at low concentrations. With pairs of dietary sterols, however, non-additive or ‘saving’ effects are sometimes seen.

It is suggested that the tu bw allele allows the resolution of several discrete developmental functions for sterols and/or their immediate metabolic products in Drosophila, which cannot synthesize its own sterol. However, the molecular nature of these functions is little understood.

Type
Research Article
Information
Genetics Research , Volume 20 , Issue 3 , December 1972 , pp. 317 - 329
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Bryant, P. J. & Sang, J. H. (1969). Physiological genetics of melanotic tumors in Drosophila melanogaster. VI. The tumorigenic effects of juvenile hormone-like substances. Genetics 62, 321–336.CrossRefGoogle ScholarPubMed
Burdette, W. J. (1954). Effects of ligation of Drosophila larvae on tumor incidence. Cancer Research 14, 780–782.Google ScholarPubMed
Burnet, B. & Sang, J. H. (1963). Physiological genetics of melanotic tumors in Drosophila melanogaster. III. Phenocritical period in relation to tumor formation in the tu bw; st su-tu strain. Genetics 49, 599–610.CrossRefGoogle Scholar
Clark, A. J. & Bloch, K. (1959). Functions of sterols in Dermestes vulpinus. J. biological Chemistry 234, 2583–2588.CrossRefGoogle ScholarPubMed
Clayton, R. B. (1964). The utilisation of sterols by insects. J. Lipid Research 5, 3–19.CrossRefGoogle ScholarPubMed
Cooke, J. & Sang, J. H. (1970). Utilization of sterols by larvae of Drosophila melanogaster. J. Insect Physiology 16, 801–812.CrossRefGoogle ScholarPubMed
Galbraith, M. N., Horn, D. H. S., Middleton, E. J. & Thomson, J. A. (1970). The biosynthesis of crustecdysone in the blowfly Calliphora stygia. Chemical Communications 3, 179–180.CrossRefGoogle Scholar
Lindsley, D. L. & Geell, E. J. (1968). Genetic variations of Drosophila melanogaster. Carnegie Institute of Washington Publication No. 627.Google Scholar
Rizki, M. T. M. (1960). Melanotic tumor formation in Drosophila. Journal Morphology 106, 147–158.CrossRefGoogle Scholar
Robbins, W. E., Kaplanis, J. N., Svoboda, J. A. & Thompson, M. J. (1971). Steroid metabolism in insects. Ann. Review Entomology 16, 53–72.CrossRefGoogle Scholar
Sang, J. H. (1956). The quantitative nutritional requirements of Drosophila melanogaster. Journal Experimental Biology 33, 45–72.CrossRefGoogle Scholar
Sang, J. H. (1966). Clearing Drosophila adults. Drosophila Information Service 41, 200.Google Scholar
Sang, J. J. (1969). Biochemical basis of hereditary melanotic tumors in Drosophila. National Cancer Institute Monograph 31, 291–301.Google ScholarPubMed
Sang, J. H. (1972). Utilisation of lipids by Drosophila melanogaster, in The Significance of Insect and Mite Nutrition (in Press).Google Scholar
Sang, J. H. & Burnet, B. (1967). Physiological genetics of melanotic tumors in Drosophila melanogaster. IV. Gene-environment interactions of the tu bw with different third chromosome backgrounds. Genetics 56, 743–754.CrossRefGoogle Scholar
Thompson, M. J., Svoboda, J. A., Kaplans, J. N. & Robbins, W. E. (1972). Metabolic pathways of steroids in insects. Proceedings Royal Society London B 180, 203–221.Google ScholarPubMed
Van't Hoog, E. G. (1935). Aseptic culture of insects in vitamin research. Zeitschrift Vitamin-forschung 5, 118–125.Google Scholar