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Phosphatases of Coprinus lagopus: the conditions for their production and the genetics of the alkaline phosphatase

Published online by Cambridge University Press:  14 April 2009

Jane North  and
D. Lewis
Jane North
Affiliation:
Department of Botany and, Microriology, University College London
D. Lewis
Affiliation:
Department of Botany and, Microriology, University College London
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Summary

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1. Coprinus lagopus produces two non-specific phosphatases: a constitutive acid phosphatase, and an alkaline phosphatase which is repressed during growth on media with a high inorganic phosphate concentration.

2. The alkaline phosphatase is also repressed when Coprinus is grown on an organic phosphate source; but if the acid phosphatase is selectively inhibited by fluoride the alkaline phosphatase is de-repressed and growth is comparable to that observed on an inorganic phosphate source.

3. Alkaline phosphatase is not repressed in aerial mycelium or sporophores even when grown on high phosphate medium.

4. Mutants altered in their capacity to synthesize alkaline phosphatase were selected from two compatible wild-type strains, H2 and H5.

5. Mutants producing a higher level of alkaline phosphatase than wild-type (‘regulator’ mutants) fall into four (or possibly five) complementation groups. Assuming five separate genes, two pairs are linked; the remaining one is independent and on another chromosome.

6. Mutants deficient in alkaline phosphatase synthesis fall into at least three groups. They were tested for linkage to ‘regulator’ loci but so far there is no evidence of this.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 2 , October 1971 , pp. 153 - 166
Copyright
Copyright © Cambridge University Press 1971

References

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