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Phenotypic suppression by streptomycin-adenylate and bluensomycin-adenylate*

Published online by Cambridge University Press:  14 April 2009

J. H. Harwood  and
David H. Smith
J. H. Harwood
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
David H. Smith
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
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An arg− conditionally Sm-dependent E. coli B strain became unable to grow on minimal agar containing low levels of Sm when the strain acquired an R factor which mediates Sm and Blu adenylation. The same R-containing strain, however, grew normally when Blu was substituted for Sm in the agar. The data strongly suggest that Sm-adenylate does not suppress the arg− phenotype in an in vivo system. The possibility that Blu-adenylate can cause phenotypic suppression without being bactericidal in vivo is discussed.

Type
Research Article
Information
Genetics Research , Volume 14 , Issue 3 , December 1969 , pp. 259 - 273
Copyright
Copyright © Cambridge University Press 1969

References

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