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Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors readily transferable between Pseudomonas and the Enterobacteriaceae

Published online by Cambridge University Press:  14 April 2009

P. M. Chandler  and
V. Krishnapillai
P. M. Chandler
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria, 3168, Australia
V. Krishnapillai
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria, 3168, Australia
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R factors have been demonstrated in multiply drug resistant strains of enterobacteria and Pseudomonas aeruginosa in a Birmingham hospital (Lowbury et al. 1969; Ingram, Richmond & Sykes, 1973). A comparative genetic analysis of these R factors has been initiated on the basis of a variety of phenotypic characteristics. This paper describes the properties of R factors derived from strains which could transfer multiple drug resistance to the recipient species P. aeruginosa, Escherichia coli, Shigella flexneri and Salmonella typhimurium. Two types of R factor could be recognized phenotypically. The single group 1 R factor, R18–1 which is probably the same as RPl-1 (Ingram et al. 1972) was different to the group 2 R factors in many respects, including host range, R factor-specific phage plating, cellular location, drug resistance pattern, and stability. The group 2 R factors were found to be very similar to RPl (Grinsted et al. 1972) and R1822 (Olsen & Shipley, 1973) with respect to their wide host range, plating of a sex specific phage, extrachromosomal location, and drug resistance pattern. Compatibility was shown between the group 1 R factor and a group 2 R factor, providing additional evidence for significant genetic differences.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 3 , June 1974 , pp. 239 - 250
Copyright
Copyright © Cambridge University Press 1974

References

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