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The phasmid as a tool for plasmid genetics: I. Fine structure of the β-lactamase gene

Published online by Cambridge University Press:  14 April 2009

Luisa Castagnoli ,
Gianni Cesareni  and
Sydney Brenner
Luisa Castagnoli
Affiliation:
MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge CB2 2QH, England
Gianni Cesareni
Affiliation:
MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge CB2 2QH, England
Sydney Brenner
Affiliation:
MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge CB2 2QH, England
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The reversible integration of a plasmid into a phage genome via int-mediated recombination permits rapid and facile analysis of plasmid genes. Seventy-six independent mutants in the β-lactamase (bla) gene have been isolated and genetically mapped. The end points of deletions generated by partial digestion with restriction enzymes have been identified and this, together with the assignment of some nonsense mutations to specific amino acid residues, allows alignment of the genetic map with the DNA sequence.

Type
Research Article
Information
Genetics Research , Volume 40 , Issue 3 , December 1982 , pp. 217 - 231
Copyright
Copyright © Cambridge University Press 1982

References

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